Unusual G and P genotypes of porcine rotavirus from Paraná and Santa Catarina states, Brazil (2001)
- Authors:
- USP affiliated authors: RACZ, MARIA LUCIA BARBOSA DE OLIVEIRA - ICB ; DURIGON, EDISON LUIZ - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Group A rotaviruses are the major etiologic agents of acute diarrhea in humans and animals. Rotavirus serotype classification is based on differences in antigenic determinants that elicit neutralizing antibodies on the major component of the outer capsid VP7 (G serotypes), and the spike protein, VP4 (P serotypes). Serotyping and genotyping characterization of rotavirus strains are important to define the diversity in circulating strains. Studies in several countries have identified at least four main serotypes of porcine rotavirus, G3, G4, G5 and G11, and two main P serotypes and genotypes, P2B [6,Gott] and P9 [7]. In Brazil only a limited number of porcine samples have been studied for their G and P type specificities. Porcine stool specimens (167) were collected from 52 small individually owned farms in the states of Paraná, Santa Catarina e Rio Grande do Sul. Fifty-nine samples (35,3%) were positive for rotavirus by enzyme immunoassay (EIARA -FIOCRUZ ).Objetives: The objective of this study was to characterize this samples by RT-PCR and gene sequenceMethods and Results: The positive samples were genotyped by reverse transcription/ polymerase chain reaction (RT-PCR), using specific primers for G1-G6 and G8- G11 and for P [1], P [4]-P [11]. Samples were characterized as common porcine G genotypes G [3], G [4] and G [5] in combination with P genotypes P [6], P [7] and mixtures of P [6] with P [7], in the same sample. Besides the published atypical P [6,Gott][6,M37]G[9] ICB2185 strain, others unusual genotypes and mixture of genotypes were also detected: one sample each with P[7]G[2][4][9] specificity, P[6,Gott] G[9], P[6,Gott] [6,M37] G[4][9], P[7] G[10] and P[7] [6,Gott] [6,M37] G[10]. Samples with genotype G[10] specificity have not been previously described in porcine samples in Brazil, but they are a common pathogen for bovines. One G[10] strain was further characterized by sequencing of VP7 gene. Preliminary results shows that deduced amino acid sequence of ICB2013 VP7 has less than 80% identity with G10 B223 bovine strain and less than 85 % identity with other G serotypes. Conclusion:This unusual combination of G and P types suggests the occurrence of natural reassortment and/or interspecies transmission of rotaviruses
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2001
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
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ABNT
KROEFF, S S et al. Unusual G and P genotypes of porcine rotavirus from Paraná and Santa Catarina states, Brazil. 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001. . Acesso em: 03 maio 2024. -
APA
Kroeff, S. S., Rácz, M. L., Durigon, E. L., & Munford, V. (2001). Unusual G and P genotypes of porcine rotavirus from Paraná and Santa Catarina states, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Kroeff SS, Rácz ML, Durigon EL, Munford V. Unusual G and P genotypes of porcine rotavirus from Paraná and Santa Catarina states, Brazil. Resumos. 2001 ;[citado 2024 maio 03 ] -
Vancouver
Kroeff SS, Rácz ML, Durigon EL, Munford V. Unusual G and P genotypes of porcine rotavirus from Paraná and Santa Catarina states, Brazil. Resumos. 2001 ;[citado 2024 maio 03 ] - Analysis of VP4 sequence of an unusual porcine rotavirus strain from southern Brazil
- Genotype characterization of group a rotavirus from São Paulo City, SP, Brazil
- Sequence analysis of atypical G genotype in bovine rotavirus samples from state of Goias, Brazil
- Diversity of G2 serotypes/genotypesof human rotavirus in São Paulo, Brazil
- Molecular characterização by gene sequence of atypical genotype G[3] bovine rotavirus sample from the state of Goiás , Brazil
- Genotype characterization of group a rotavirus from São Paulo City, SP, Brazil
- Molecular characterization of porcine rotaviruses from southern region of brazil: Characterization of an atypical genotype G[9] strain
- Evidence of a new rotavirus p genotype in porcine samples from southern Brazil
- Molecular characterization by gene sequence of G5 and G9 human rotavirus samples
- Molecular characterization by gene sequence of atypical genotype G[3] bovine rotavirus sample from the state of Goiás, Brazil
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