Sequence analysis of an atypical P[8] bovine rotavirus sample from state of Goiás, Brazil (2002)
- Authors:
- USP affiliated authors: RACZ, MARIA LUCIA BARBOSA DE OLIVEIRA - ICB ; DURIGON, EDISON LUIZ - ICB ; MUNFORD, VERIDIANA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Rotaviruses are the major etiologic agents of acute dehydrating diarrhea in many mammalian species, including calves. Members of the family Reoviridae, they have a genome consisting of 11 segments of dsRNA, each one coding for at least one protein. Two structural proteins located in the outer capsid (VP4 and VP7) are used for molecular characterization of G and P genotypes by RT-PCR and by genome sequencing. BoRV-A sample ICB896, characterized as G3Pnegative by RT-PCR using specific primers for animal and human genotypes, had its VP4 gene partially sequenced in an ABI-Prism 377 DNA Sequencer, with Big Dye kit. Nucleotide sequence analysis showed that ICB896 had a homology of 90,1% with P[8]HuRV-A/WA and that at the P[8]-specific primer binding position (339-356) 5 mismatches were present, which could have prevented primer 1T.1 from binding to ICB896 during RT-PCR. These results suggest that molecular characterization of BoRV-A by RT-PCR can lead to non-characterized genotypes due to differences in the nucleotide sequence of the primer-binding site, and that gene sequencing then become important for more precise characterizations. Also, a BoRV-A sample characterized as a HuRV-A P genotype may suggest inter-species transmission of this virus. This finding may contribute for the development of more efficient and specific bovine rotavirus vaccines worldwide
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2002
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
CARUZO, T A R et al. Sequence analysis of an atypical P[8] bovine rotavirus sample from state of Goiás, Brazil. 2002, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. . Acesso em: 21 maio 2024. -
APA
Caruzo, T. A. R., Munford, V., Brito, W. M. E. D., Kroefs, S. S., Durigon, E. L., & Racz, M. L. B. de O. (2002). Sequence analysis of an atypical P[8] bovine rotavirus sample from state of Goiás, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Caruzo TAR, Munford V, Brito WMED, Kroefs SS, Durigon EL, Racz MLB de O. Sequence analysis of an atypical P[8] bovine rotavirus sample from state of Goiás, Brazil. Resumos. 2002 ;[citado 2024 maio 21 ] -
Vancouver
Caruzo TAR, Munford V, Brito WMED, Kroefs SS, Durigon EL, Racz MLB de O. Sequence analysis of an atypical P[8] bovine rotavirus sample from state of Goiás, Brazil. Resumos. 2002 ;[citado 2024 maio 21 ] - Identification, genotyping and nucleotide sequencing of norwalk-like virus in stool samples of children with gastroenteritis, from São Paulo city, Brazil
- Sequence analysis of the VP7 gene of atypical bovine and porcine rotavirus samples from Brazil
- Analysis of VP4 sequence of an unusual porcine strain from southern Brazil
- Molecular characterization by gene sequence of G5 and G9 human rotavirus samples
- Diversity of G2 serotypes/genotypes of human rotavirus in São Paulo, Brazil
- Sequence analysis of P genotype mixtures in bovine rotavirus saples from state of Goiás, Brazil
- Evidence of a new rotavirus P genotype in porcine samples from southern Brazil
- Sequence analysis of atypical G genotype in bovine rotavirus samples from state of Goiás, Brazil
- Electrophoretic and serological characterization of bovine rotavirus samples from an outbreak of diarrhea in a dairy farm in state of goias, brazil
- Gastroenterites virais
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
