Sequence analysis of atypical G genotype in bovine rotavirus samples from state of Goiás, Brazil (2002)
- Authors:
- USP affiliated authors: RACZ, MARIA LUCIA BARBOSA DE OLIVEIRA - ICB ; DURIGON, EDISON LUIZ - ICB ; MUNFORD, VERIDIANA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Rotaviruses are the major etiologic agents of acute dehydrating diarrhea in many mammalian species, including calves. Members of the family Reoviridae, they have a genome consisting of 11 segments of dsRNA, each one coding for at least one protein. Two structural proteins located in the outer capsid (VP4 and VP7) are used for molecular characterization of G and P genotypes, by reverse transcription followed by polymerase chain reaction (RT-PCR). BoRV samples ICB1061 and ICB1064, from Piracanjuba and ICB743, from Hidrolândia, Goiás, Brazil, were previously characterized by RT-PCR as mixtures of genotypes G3+G6 and G3+G6+G10, respectively. ICB991 sample from Piracanjuba could not be characterized by RT-PCR. To investigate these atypical findings, samples had their VP7 gene partially sequenced in an ABI-Prism 377 DNA Sequencer, with Big Dye kit and the sequences were analyzed with DNAstar software. Preliminary results show that nucleotide sequences of ICB1061 and ICB1064 have an identity of 94,7% and 94,5% with G6-BoRV/UK, 92,7% and 92,5% with G6-BoRV/NCDV and less than 74% and 83,3% identity with several G3 strains, respectivelyNucleotide sequence analysis of ICB1061 and ICB1064 shows that, at the G3-specific primer binding position (689-709) 5 mismatches are present. ICB743 nucleotide sequence have a 92,5% identity with G6-BRV/UK and less than 75,1% with G3 and G10 strains. ICB991 sample had it VP7 gene sequenced and its nucleotide sequence showsidentities of 93,1% to G6-BoRV/UK and 91% to G6-BoRV/NCDV. Only two mismatches are found at the G6-specific primer binding position (499-481). These samples will have their VP7 gene totally sequenced after cloning of PCR products for more complete results. So far, results show that molecular characterization of BoRV-A by RT-PCR using HuRV-A specific primers can lead to mistakes or non-characterized genotypes due to very little differences in the nucleotide sequence of the primer-binding site. The molecular characterization by gene sequence then becomes very important for more precise characterizations and also for phylogenetic analysis of rotaviruses strains
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2002
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
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ABNT
CARUZO, T A R et al. Sequence analysis of atypical G genotype in bovine rotavirus samples from state of Goiás, Brazil. 2002, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. . Acesso em: 21 maio 2024. -
APA
Caruzo, T. A. R., Munford, V., Brito, W. M. E. D., Durigon, E. L., & Racz, M. L. B. de O. (2002). Sequence analysis of atypical G genotype in bovine rotavirus samples from state of Goiás, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Caruzo TAR, Munford V, Brito WMED, Durigon EL, Racz MLB de O. Sequence analysis of atypical G genotype in bovine rotavirus samples from state of Goiás, Brazil. Resumos. 2002 ;[citado 2024 maio 21 ] -
Vancouver
Caruzo TAR, Munford V, Brito WMED, Durigon EL, Racz MLB de O. Sequence analysis of atypical G genotype in bovine rotavirus samples from state of Goiás, Brazil. Resumos. 2002 ;[citado 2024 maio 21 ] - Sequence analysis of an atypical P[8] bovine rotavirus sample from state of Goiás, Brazil
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