Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil (2002)
- Authors:
- USP affiliated authors: RACZ, MARIA LUCIA BARBOSA DE OLIVEIRA - ICB ; MUNFORD, VERIDIANA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Acute infantile diarrhea is responsible for a large number of pediatric hospitalizations worldwide and for high infant mortality in developing countries. Rotavirus is the most frequent pathogen associated with diarrhea in young children. In the present study we tested 47 rotavirus positive samples collected in the Hospital Infantil Lucídio Portela, Teresina, Piauí, from children 0 to 5 years of age with diarrhea. Rotavirus was identified in stools by an enzyme immunoassay (EIARA-FIOCRUZ). Samples were then submitted to molecular characterization, using reverse transcription (RT) followed by polymerase chain reaction (PCR) with specific primers for the most common human and animals rotavirus genotypes. PCR results showed a high diversity of G and P genotypes. Samples were classified into common human G genotypes G1, G2 and G4, with the majority of samples (25,5%) belonging to genotype G1. Genotype G5, commonly found in porcine samples, was detected in 10,6% of the samples. Mixtures of G5 with G1, G2, G4 and G9 were also detected. The most common P genotype was P[8] (34,1%). P[4] was detected in 4,3% of the samples. Mixed P genotype P[1]+P[8] was detected in 8,5% of the samples and P[6,M37]+P[8], P[6,Gott]+P[8] and P[4]+P[6,M37]+P[6,Gott] were also found. P[6] genotypes were designated as P[6,M37] (human specific) and P[6,Gott] (porcine specific). G5 strains are common human pathogens in Brazil, usually combined with P[8] genotype. In the present study we foundG5 and several mixtures of G5 with G1, G2, G4 and G9 combined with P[1]+P[8], P[8], P[6,M37]+P[8] and P[6,Gott]+P[8]. The majority of G1 were combined with P[8] as expected but combinations with P[4] and P[6, M37]+P[8] were also detected. P[1] genotype is commonly found in bovine samples. Mixtures of genotypes suggest the possibility of exchange of VP7/ VP4 genes between animal and human rotaviruses and interspecies transmission of these viruses
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2002
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
MACEDO, C I et al. Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil. 2002, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. . Acesso em: 21 maio 2024. -
APA
Macedo, C. I., Christofoletti, A., Munford, V., & Racz, M. L. B. de O. (2002). Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Macedo CI, Christofoletti A, Munford V, Racz MLB de O. Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil. Resumos. 2002 ;[citado 2024 maio 21 ] -
Vancouver
Macedo CI, Christofoletti A, Munford V, Racz MLB de O. Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil. Resumos. 2002 ;[citado 2024 maio 21 ] - Electrophoretic and serological characterization of bovine rotavirus samples from an outbreak of diarrhea in a dairy farm in state of goias, brazil
- Gastroenterites virais
- Ocorrencia de rotavirus em bezerros neonatos provenientes de rebanhos leiteiros do municipio de piracanjuba, goias
- Serological and genotypical characterization of group a rotavirus samples from children of Goiania-Goias, Brazil
- Detection and molecular caracterization of G and P genotypes of human rotavirus from São Paulo, Brazil
- Molecular characterization of astrovirus in stool samples from children in São Paulo, Brazil
- Identificacao, cultivo e genotipagem de rotavirus suinos em culturas celulares
- Serological and electrophoretic characterization of bovine rotavirus identified in dairy herds from district of piracanjuba, Goias
- Characterization of brazilian deer (Blastocerus dichotomus) rotavirus as G10 genotype by RT-PCR
- Molecular characterization of bovine rotavirus from São Paulo state, Brazil
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas