Construção de dois adenovírus recombinantesque expressam a proteína do nucleocapsídeo e a fosfoproteína do vírus sincicial respiratório humano (HRSV) (2003)
- Authors:
- Autor USP: VENTURA, ARMANDO MORAIS - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Português
- Abstract: Human respiratory syncytial virus (HRSV) is an enveloped nonsegmented negative-sense RNA virus that belongs to the Paramyxoviridae family. Its genome has approximately 15200 nucleotides encoding 11 proteins. N protein is responsible for the encapsidation of the viral genome, forming a helical nucleocapsid which is the template for replication and transcription of the genome. P protein functions as a molecular chaperon for N and is responsible for the delivery of N to assembling nucleocapsids. HRSV is a highly infectious virus that is the leading cause of severe respiratory tract disease in young infants worldwide. The aim of this work was to construct two recombinant adenoviruses carrying separately the genes of nucleocapsid protein (N) and phosphoprotein (P) from HRSV, using Adeno-X Expression System (Clontech). In order to get the recombinant viruses, we did RT-PCR of RNA extracted from the HRSV A2 strain, cloned the genes (N and P) into pCR-XL-TOPOs and sequenced the plasmids to assure the correct cloning. Then we sub-cloned the genes into pShuttle vectors and after that into pAdeno-X vectors from the Adeno-X kit. pAdeno-X vectors were transfected into HEK 293 cells which produced the recombinant viruses
- Imprenta:
- Publisher: Universidade de São Paulo
- Publisher place: São Paulo
- Date published: 2003
- Source:
- Título do periódico: Resumos
- Conference titles: Simpósio International de Iniciação Científica da Universidade de São Paulo, SIICUSP
-
ABNT
GIMENES, Manuela Vieira et al. Construção de dois adenovírus recombinantesque expressam a proteína do nucleocapsídeo e a fosfoproteína do vírus sincicial respiratório humano (HRSV). 2003, Anais.. São Paulo: Universidade de São Paulo, 2003. . Acesso em: 21 maio 2024. -
APA
Gimenes, M. V., Ventura, A. M., Simabuco, F. M., Kosaka, I. M., & Carromeu, C. (2003). Construção de dois adenovírus recombinantesque expressam a proteína do nucleocapsídeo e a fosfoproteína do vírus sincicial respiratório humano (HRSV). In Resumos. São Paulo: Universidade de São Paulo. -
NLM
Gimenes MV, Ventura AM, Simabuco FM, Kosaka IM, Carromeu C. Construção de dois adenovírus recombinantesque expressam a proteína do nucleocapsídeo e a fosfoproteína do vírus sincicial respiratório humano (HRSV). Resumos. 2003 ;[citado 2024 maio 21 ] -
Vancouver
Gimenes MV, Ventura AM, Simabuco FM, Kosaka IM, Carromeu C. Construção de dois adenovírus recombinantesque expressam a proteína do nucleocapsídeo e a fosfoproteína do vírus sincicial respiratório humano (HRSV). Resumos. 2003 ;[citado 2024 maio 21 ] - Production of polyclonal antibodies against the human respiratory syncytial virus nucleoprotein and phosphoprotein expressed in Escherichia coli
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