Artigo de periodico

Quantification of fecal estradiol and progesterone metabolites in syrian hamsters (Mesocricetus auratus) (2005)

• Authors:
  • Chelini, Marie-Odile Monier
  • Souza, Nívea Lopes de
  • Oliveira, Cláudio Alvarenga de
  • Rocha, A. M
  • Felippe, Érika Cristiane Gutierrez
• USP affiliated authors: SOUZA, NIVEA LOPES DE - FMVZ ; OLIVEIRA, CLAUDIO ALVARENGA DE - FMVZ ; FELIPPE, ERIKA CRISTIANE GUTIERREZ - FMVZ
• Unidade: FMVZ
• DOI: 10.1590/s0100-879x2005001100021
• Subjects: HAMSTERS; ENDOCRINOLOGIA VETERINÁRIA; ESTRÓGENOS; RADIOIMUNOENSAIO; REPRODUÇÃO ANIMAL
• Language: Inglês
• Abstract: Altemative methods to the utilization of laboratory animal blood and its by-products are particularly attractive, especially regarding hamsters due to their small size and difficulties in obtaining serial blood samples. Steroid hormone metabolite quantification in feces, widely used in studies of free-ranging or intractable animals, is a noninvasive, non-stressor, econornical, and animal saving technique which allows longitudinal studies by perrnitting frequent sampling of the same individual. The present study was undertaken to determine the suitability of this method for laboratory animals. Estradiol and progesterone metabolites were quantified by radioimmunoassay in feces of intact, sexually mature female Syrian harnsters during the estrous cyc1e (control) and in feres of superovulated females. Metabolites were extracted by fecal dilution in ethanol and quantified by solid phase radioimmunoassay. Median estroge;. and progesterone concentrations were 9.703 and 180.74 ng/g feces in the control group, respectively. Peaks of estrogen (22.44:t 4.54 ng/g feces) and progesterone (655.95:t 129.93 ng/g feres) mean fecal concentrations respectivelyoccurred 12 h before and immediately after ovulation, which is easily detected in this species by observation of a characteristic vaginal postovulatory discharge. Median estrogen and progesterone . concentrations (28.159 and 586.57 ng/g feres, respectively) were significantly higher in superovulated animal feces (P <0.00(1). The present study demonstrated that it is possible to monitor ovarian activity in Syrian harnsters non-invasively by measuring fecal estradiol and progesterone metabolites. This technique appears to be a quite encouraging method for the development of new endocrinologic studies on laboratory animals.
• Imprenta:
  • Publisher place: Ribeirão Preto
  • Date published: 2005
• Source:
  • Título do periódico: Brazilian Journal of Medical and Biological Research
  • ISSN: 0100-879X
  • Volume/Número/Paginação/Ano: v. 38, n. 11, p. 1711-1717, 2005

Informações sobre o DOI: 10.1590/s0100-879x2005001100021 (Fonte: oaDOI API)
• Este periódico é de acesso aberto
• Este artigo é de acesso aberto
• URL de acesso aberto
• Cor do Acesso Aberto: gold
• Licença: cc-by

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How to cite

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• ABNT

  CHELINI, Marie-Odile Monier et al. Quantification of fecal estradiol and progesterone metabolites in syrian hamsters (Mesocricetus auratus). Brazilian Journal of Medical and Biological Research, v. 38, n. 11, p. 1711-1717, 2005Tradução . . Disponível em: https://doi.org/10.1590/s0100-879x2005001100021. Acesso em: 06 maio 2024.

• APA

  Chelini, M. -O. M., Souza, N. L. de, Oliveira, C. A. de, Rocha, A. M., & Felippe, É. C. G. (2005). Quantification of fecal estradiol and progesterone metabolites in syrian hamsters (Mesocricetus auratus). Brazilian Journal of Medical and Biological Research, 38( 11), 1711-1717. doi:10.1590/s0100-879x2005001100021

• NLM

  Chelini M-OM, Souza NL de, Oliveira CA de, Rocha AM, Felippe ÉCG. Quantification of fecal estradiol and progesterone metabolites in syrian hamsters (Mesocricetus auratus) [Internet]. Brazilian Journal of Medical and Biological Research. 2005 ; 38( 11): 1711-1717.[citado 2024 maio 06 ] Available from: https://doi.org/10.1590/s0100-879x2005001100021

• Vancouver

  Chelini M-OM, Souza NL de, Oliveira CA de, Rocha AM, Felippe ÉCG. Quantification of fecal estradiol and progesterone metabolites in syrian hamsters (Mesocricetus auratus) [Internet]. Brazilian Journal of Medical and Biological Research. 2005 ; 38( 11): 1711-1717.[citado 2024 maio 06 ] Available from: https://doi.org/10.1590/s0100-879x2005001100021

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