The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line (2005)
- Authors:
- Autor USP: JAEGER, RUY GASTALDONI - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Inglês
- Abstract: Objectives: Adenoid cystic carcinoma is a malignant salivary gland neoplasm with high level of recurrence and metastasis. We have demonstrated that laminin-1 modulates the phenotype of an adenoid cystic carcinoma (CAC2) cell line. We are currently studying the expression of a non-integrin laminin receptor-the 32/67kDa laminin-binding protein (LBP)- in adenoid cystic carcinoma. Methods and results: Immunohistochemistry showed LBP in adenoid cystic carcinoma in vivo. Immunofluorescence of CAC2 cells also revealed this receptor. The LBP binds a sequence of laminin _eta 1 chain, the YIGSR peptide. This peptide promotes cell migration. We decided to study the role played by the peptide YIGSR and its ligand, the LBP receptor, in CAC2 cells. These cells were grown inside either laminin-1 (controls) or laminin-1 enriched with YIGSR (treated). Light microscopy showed that laminin-1 induced CAC2 cells to create solid formations of cells closely packed. CAC2 cells grown in contact with YIGSR were non-cohesive and spindle shaped. To address whether the effect of YIGSR was dependent on LBP, CAC2 cells were pretreated with antibody against this receptor. Blockage of LBP receptor inhibited YIGSR effect on CAC2 morphology. We then decided to study whether YIGSR would inhibit the presence of adhesion molecules in CAC2 cells. We prepared cytoskeletal fractions of CAC2 cells grown either inside laminin-1 or laminin-1 enriched with YIGSR. Western blot of these fractions demonstrated thatYIGSR decreased the amount of E-cadherin and beta-catenin in CAC2 cells, when compared to cells grown inside laminin-1. Pretreatment of CAC2 cells wit anti-LBP restored e-cadherin and beta catenin expression. Conclusion: YIGSR and its ligand, 32/67kDa LBP modulates adhesion molecules in CAC2 cells
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
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ABNT
OLIVEIRA, Elaine Cyreno et al. The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line. 2005, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005. . Acesso em: 01 maio 2024. -
APA
Oliveira, E. C., Freitas, V. M., Gama-de-Souza, L. N. da, Araújo, V. C., Furuse, C., & Jaeger, R. G. (2005). The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Oliveira EC, Freitas VM, Gama-de-Souza LN da, Araújo VC, Furuse C, Jaeger RG. The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line. Resumos. 2005 ;[citado 2024 maio 01 ] -
Vancouver
Oliveira EC, Freitas VM, Gama-de-Souza LN da, Araújo VC, Furuse C, Jaeger RG. The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line. Resumos. 2005 ;[citado 2024 maio 01 ] - The effect of laminin and its SIKVAV peptide on myoepithelioma phenotype
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