Characterization of NOD-like genes in Leishmania (2005)
- Authors:
- Autor USP: ULIANA, SILVIA RENI BORTOLIN - ICB
- Unidade: ICB
- Assunto: PARASITOLOGIA
- Language: Inglês
- Abstract: Many of the proteins in the NOD family have been implicated in the intracellular recognition of pathogens. The function of NOD proteins is dependent on the binding and cleavage of nucleosides through domains known as Walker A and B (Nuñez et al., Annu. Rev. Biochem. 74:355-83, 2005). In the process of characterizing the genomic region of the L. amazonensis meta 1 gene (FEMS Microbiol Lett, 238(1):213-9, 2004), we identified an ORF of 2,03 Kb that presented similarity with the human NOD3 protein. Objective: Characterization of NODlike genes in Leishmania. Methods and results: Northern blots indicated that this gene is upregulated in amastigotes of L. amazonensis. The complete nucleotide sequence of the L. amazonensis ORF was obtained. The predicted translated sequence of 675 amino acids was used to identify orthologous L. major and L. infantum proteins (www.genedb.org).The alignment of these sequences revealed high degrees of similarity. The 73-kDa recombinant NOD-like protein was expressed and used to raise specific antibodies that recognized a 45 kDa protein in total extracts of L. amazonensis amastigotes. The search for orthologues on T. brucei and T. cruzi translated genome databanks identified predicted proteins of 478 and 470 amino acids, respectively, with high degrees of similarity with the L. amazonensis protein. The sequence alignment of the 3 peptides showed that T. brucei and T. cruzi proteins lack the C-terminal regionof the NOD-like protein found in the Leishmania species. A sequence search in the unfinished L. major Friedlin genome project data allowed the identification of 5 further possible NOD-related genes. One of these genes (LmjF 11.0890) encodes a protein containing Walker's A and B domains. The characterization of this gene and of the encoded protein is under way. Conclusion: The characterisation of NOD-like genes in Leishmania may produce the necessary tools to investigate the participation of these proteins in the macrophage-parasite interaction.
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
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ABNT
FRANCO, F. A. L. e CRISPIM, A. F. e ULIANA, Silvia Reni Bortolin. Characterization of NOD-like genes in Leishmania. 2005, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005. . Acesso em: 06 maio 2024. -
APA
Franco, F. A. L., Crispim, A. F., & Uliana, S. R. B. (2005). Characterization of NOD-like genes in Leishmania. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Franco FAL, Crispim AF, Uliana SRB. Characterization of NOD-like genes in Leishmania. Resumos. 2005 ;[citado 2024 maio 06 ] -
Vancouver
Franco FAL, Crispim AF, Uliana SRB. Characterization of NOD-like genes in Leishmania. Resumos. 2005 ;[citado 2024 maio 06 ] - Characterization of Tbmeta1 and 2, T. brucei homologues of the Leishmania infective-stage specific meta proteins.
- Mapping and characterization of the Leishmania (L.) amazonensis genomic region containing the meta 1 gene:: comparative study with the L. (L.) major genome.
- Characterization of nod-like genes in Leishmania
- The meta 2 gene of Leishmania: structural characterisation and expression
- Identification of sequences related to the meta 1 gene in trypanosomatids
- Structure and function of a stage-regulated gene in Leishmania
- Caracterização molecular de uma proteína rica em sequencias repetidas de leucina em Leishmania braziliensis
- In Vitro and In Vivo miltefosine susceptibility of a Leishmania amazonensis isolate from a patient with diffuse cutaneous leishmaniasis
- Ros3 (Lem3p/CDC50) gene dosage is implicated in miltefosine susceptibility in Leishmania (Viannia) braziliensis clinical isolates and in Leishmania (Leishmania) major
- Route map for the discovery and pre-clinical development of new drugs and treatments for cutaneous leishmaniasis
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