Which is the feature of NK1.'1 POT.+' T cells responding to acute Plasmodium chabaudi malaria? (2006)
- Authors:
- USP affiliated authors: MOSIG, JOSE MARIA ALVAREZ - ICB ; LIMA, MARIA REGINA D'IMPERIO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives : Plasmodium infection results in activation of T, NKT and NK lymphocytes, which has been implied in the initial control of parasitemia, through IFN- ã - mediated effector mechanisms. NKT cells can be distinguished by the expression of NK cell markers such as CD161 (NK1.1), a molecule involved in cell activation, IFN-ã production and cytotoxicity. However, NKT cells represent a heterogeneous population since not all CD1drestricted T cells express NK1.1 and some NK1.1+ T cells are not restricted by CD1d. Moreover, conventional T cells increase NK1.1 expression when activated, whereas a decrease is observed in CD1d-restricted T cells. Thus, this study aims to characterize NK1.1+ T cells in the acute response to P. chabaudi. Methods and Results : M ultiparametric flow cytometric analysis of splenocytes obtained from C57BL/6 mice on days 0, 4 and 7 of infection with 106 P. chabaudi parasitized erythrocytes revealed an increase of CD3+NK1.1+ cell frequency at the different time points, being the total number of these cells higher on day 7.Among CD3+NK1.1+ cells, CD4+, CD8+ and CD4-CD8- cells represented 35,83%, 21,38% and 42,79%, respectively. Numbers similar to those were found in infected CD1dKO mice. CD4+NK1.1+ and CD8+NK1.1+ cells showed an activated phenotype (CD69 high, CD45RBhigh, CD44high, and CD62Lhigh) and produced IFN-ã. A higher expression of Fas and FasL was detected in CD8+NK1.1+ cells at day 7 ofinfection. Analysis of CFSE-labeled cells kept in culture for 72h showed lower spontaneous proliferation of NK1.1+ cells compared to NK1.1- cells, for both CD4+ and CD8+ lymphocytes. When stimulated with plate-bound anti-CD3 antibodies, a strong proliferative response occurred at day 0, decreasing after infection with NK1.1+ cells showing the lowest scores. Conclusion: These results suggest that CD4+NK1.1+ and CD8+NK1.1+ cells have effector functions that may contribute to the acute immune response to P. chabaudi. Keywords : Plasmodium chabaudi, T lymphocytes, NK1.1 molecule.
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
MUXEL, S M et al. Which is the feature of NK1.'1 POT.+' T cells responding to acute Plasmodium chabaudi malaria? 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 03 maio 2024. -
APA
Muxel, S. M., Rosário, A. P. F., Sardinha, L. R., Alvarez, J. M. M., & Lima, M. R. D. 'I. (2006). Which is the feature of NK1.'1 POT.+' T cells responding to acute Plasmodium chabaudi malaria? In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Muxel SM, Rosário APF, Sardinha LR, Alvarez JMM, Lima MRD'I. Which is the feature of NK1.'1 POT.+' T cells responding to acute Plasmodium chabaudi malaria? Abstracts. 2006 ;[citado 2024 maio 03 ] -
Vancouver
Muxel SM, Rosário APF, Sardinha LR, Alvarez JMM, Lima MRD'I. Which is the feature of NK1.'1 POT.+' T cells responding to acute Plasmodium chabaudi malaria? Abstracts. 2006 ;[citado 2024 maio 03 ] - Enhanced systemic and secreted antibody responses in mice submitted to a combined immunization protocol based on parental (priming) DNA vaccine and oral (boosting) delivered salmonella vaccine strain
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