Complement is necessary for full human monocyte differentiation into dendritic cells (2006)
- Authors:
- USP affiliated authors: BARBUTO, JOSE ALEXANDRE MARZAGAO - ICB ; ISAAC, LOURDES - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: Dendritic cells (DCs) and complement are important components of innate immunity; both participate in maintenance of tolerance and link innate and acquired immunity. Considering that DCs are present at most tissues and inflammatory sites and that they are a source of several complement proteins, we investigated a possible role for complement in the differentiation of human monocytes into DCs. Methods and Results: DCs were generated in vitro from leukocytes obtained by leukapheresis from healthy donors. Adherent peripheral blood mononuclear cells were isolated and cultured in the presence of GMCSF plus IL-4 in culture medium supplemented with normal human serum (DC-SHN) or C3 deficient serum (DC-C3D). DCs obtained by day 7 were CD14-CD1ahigh, CD11c+HLA-DR+ and had typical DC morphology. For the activation of DCs, LPS was added in the last 24 h of culture, resulting in an increase in the expression of co-stimulatory molecules. When compared to DCs-C3D, DCs-SHN presented a higher expression of CD1a [MFI: 43 (DCs-SHN) x 29 (DCs-C3D)], HLA-DR (MFI: 179 x 122), CD209 (MFI: 315 x 202) and of the co-stimulatory molecules, CD80 (MFI: 105 x 67) and CD86 (MFI: 135 x 85) as observed by flow cytometry.Furthermore, DCs-SHN also secreted higher levels of IL-6 [mean concentration: 110 (DCs-SHN) x 58 pg/ml (DCs-C3D)] (detected by ELISA), but similar levels of IL-10, IL-12p40 and IL-12p70. The expression of CD11c, CD205,CD83, CR1 and CD18 was not significantly different between the 2 groups. LPS stimulation of DCs induced lower expression of CD1a (MFI: 26 x 36), HLA-DR (MFI: 166 x 99) and CD209 (MFI: 184 x 121) in DCs-C3D, which also produced less IL-12p70 (23 x 100 pg/ml) than DCs cultivated in the presence of C3 (DCs-SHN). Conclusion: Our results suggest a participation of complement in DC differentiation. We are now investigating if complement- dependent processes contribute to DC activation of T cells and in the modulation of the immune response.
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
REIS, E S e BARBUTO, José Alexandre Marzagão e ISAAC, Lourdes. Complement is necessary for full human monocyte differentiation into dendritic cells. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 02 maio 2024. -
APA
Reis, E. S., Barbuto, J. A. M., & Isaac, L. (2006). Complement is necessary for full human monocyte differentiation into dendritic cells. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Reis ES, Barbuto JAM, Isaac L. Complement is necessary for full human monocyte differentiation into dendritic cells. Abstracts. 2006 ;[citado 2024 maio 02 ] -
Vancouver
Reis ES, Barbuto JAM, Isaac L. Complement is necessary for full human monocyte differentiation into dendritic cells. Abstracts. 2006 ;[citado 2024 maio 02 ] - Human monocyte-derived dendritic cells produce complement proteins
- Human monocyte-derived dendritic cells are a source of several complement proteins
- Impaired dendritic cell differentiation and maturation in the absence of C3
- Impaired dendritic cell differentiation and maturation in the absence of C3
- Complement components, regulators and receptors are produced by human monocyte-derived dendritic cells
- Complement components, regulators and receptors are produced by human monocyte-derived dendritic cells
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