Effect of vascular endothelial growth factor receptors inhibition on tumor angiogenesis in head and neck squamous cell carcinoma (2007)
- Authors:
- Autor USP: OLIVEIRA, DENISE TOSTES - FOB
- Unidade: FOB
- Subjects: CARCINOMA DE CÉLULAS ESCAMOSAS; CABEÇA; PESCOÇO; ENDOTÉLIO VASCULAR
- Language: Inglês
- Abstract: The Vascular Endothelial Growth Factor (VEGF) has been considered the most important mediator in inducing tumor angiogenesis and binds to Vascular Endothelial Growth Factor Receptor (VEGFR). PTK787/ZK 222584 (PTK/ZK) is a potent antiangiogenic tyrosine kinase VEGFR inhibitor. To understand the antiangiogenic VEGF/VEGFR pathway of PTK/ZK in squamous cell carcinoma (SCC) of the head and neck region, it was developed in vitro and in vivo studies evaluating Bcl-2, CXCL-8 and CXCL-1 expression. Immunodeficient mice recieved implants of tumor and/or endothelial cells and were treated with PTK/ZK. Tumor progression was evaluated by bioluminescence imaging. Three µm sections were obtained from paraffin embedded implants for microvessel density analisis and 7 µm sections for laser microdissection and tumor and endothelial cell RNA retrieving analyzed by RT-PCR and Real time PCR. In vitro studies with co-culture of tumor and endothelial cells were analyzed by RT-PCR, Western Blot and ELISA to evaluate Bcl-2, CXCL-8 and CXCL-1 expression. The in vitro and in vivo results showed important downregulation of Bcl-2 as well as CXCL-8 expression in endothelial cells when treated with PTK/ZK. It was also observed a significant reduction of microvessel density in animals treated with the drug. These results suggest that the antiangiogenic mechanism of PTK/ZK on endothelial cells seems to be by downregulation of Bcl-2 leading to downregulation of CXCL-8, resulting in less tumor growth.In conclusion, PTK/ZK seems to be a promising antiangiogenic drug for SCC adjuvant therapy
- Imprenta:
- Source:
- Título do periódico: Brazilian Oral Research
- ISSN: 1806-8324
- Volume/Número/Paginação/Ano: v. 21, p. 28, res. H043, 2007. Supplement 1
- Conference titles: Reunião Anual da Sociedade Brasileira de Pesquisa Odontológica - SBPqO
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ABNT
MIYAZAWA, Marta et al. Effect of vascular endothelial growth factor receptors inhibition on tumor angiogenesis in head and neck squamous cell carcinoma. Brazilian Oral Research. São Paulo: Faculdade de Odontologia de Bauru, Universidade de São Paulo. . Acesso em: 03 maio 2024. , 2007 -
APA
Miyazawa, M., Dong, Z., Zhang, Z., Neiva, K. G., Cordeiro, M. M. R., Oliveira, D. T., & Nör, J. E. (2007). Effect of vascular endothelial growth factor receptors inhibition on tumor angiogenesis in head and neck squamous cell carcinoma. Brazilian Oral Research. São Paulo: Faculdade de Odontologia de Bauru, Universidade de São Paulo. -
NLM
Miyazawa M, Dong Z, Zhang Z, Neiva KG, Cordeiro MMR, Oliveira DT, Nör JE. Effect of vascular endothelial growth factor receptors inhibition on tumor angiogenesis in head and neck squamous cell carcinoma. Brazilian Oral Research. 2007 ; 21 28.[citado 2024 maio 03 ] -
Vancouver
Miyazawa M, Dong Z, Zhang Z, Neiva KG, Cordeiro MMR, Oliveira DT, Nör JE. Effect of vascular endothelial growth factor receptors inhibition on tumor angiogenesis in head and neck squamous cell carcinoma. Brazilian Oral Research. 2007 ; 21 28.[citado 2024 maio 03 ] - Manifestações bucais iniciais das leucemias agudas
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