Fungal evaluation of rice samples purchased from market of São Paulo city (2008)
- Authors:
- Autor USP: CORREA, BENEDITO - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: There are some reports that demonstrated that rice has been contaminated with toxigenic fungi. The present study analyzed the rice samples purchased at the Brazilian market of São Paulo city. The rice groups were denominated as: husked A, unhusked A, husked B, unhusked B, Thailand rice and polished rice, portioned in 5 units per group contained 100 g. The samples were analyzed being thoroughly mixed with 90 mL of sterile distilled water and shaken in sterile tubes for 30 min. For enumeration of total fungi (moulds and yeasts) was performed using the plate method and the samples were seeded in duplicates in a serial dilution from 10-1 to 10-6 with a suspension of 0.1 ml of the dilutions in dichloran 18% glycerol agar (DG 18) and potato dextrose agar (PDA). After incubation at 25ºC for 7 days, the counting was expressed in colony forming units per gram (cfu/g). The isolation of rice grains was performed by direct plating for each dose (Berjak, 1984). Thirty-three grains of control group were surface disinfected using 0.4 % sodium hypochlorite solution during three minutes and rinsed three times with sterile distilled water and 11 grains were placed at random in each of the Petri-dishes containing DG 18 agar (in two replicates). To verify the superficial fungal contamination, the control sample of unhusked rice B was portioned in two samples, B-I and B-II. The samples B-I were not disinfected with sodium hypochlorite solution and the rice B-II samples weredisinfected. The dishes were incubated at 25ºC and examined daily for 7 days. The fungal counting was made by frequency (%) on rice grains. The present study demonstrated that the total fungal counts varied in the range 1.0 x102 - 5.0 x105 cfu/g. Genera of toxigenic fungi, such as Aspergillus spp. and Penicillium spp., were observed in all samples (except the polished rice) and the samples showed high fungal contamination.
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2008
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas da Universidade de São Paulo
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ABNT
AQUINO, S. et al. Fungal evaluation of rice samples purchased from market of São Paulo city. 2008, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2008. . Acesso em: 06 maio 2024. -
APA
Aquino, S., Nunes, T. C., Rogovschi, V. D., Zorzete, P., Reis, T. A., Gonçalez, E., et al. (2008). Fungal evaluation of rice samples purchased from market of São Paulo city. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Aquino S, Nunes TC, Rogovschi VD, Zorzete P, Reis TA, Gonçalez E, Braghini R, Corrêa B. Fungal evaluation of rice samples purchased from market of São Paulo city. Resumos. 2008 ;[citado 2024 maio 06 ] -
Vancouver
Aquino S, Nunes TC, Rogovschi VD, Zorzete P, Reis TA, Gonçalez E, Braghini R, Corrêa B. Fungal evaluation of rice samples purchased from market of São Paulo city. Resumos. 2008 ;[citado 2024 maio 06 ] - Ochratoxina em graos de milho: influencia da temperatura e da umidade relativa
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