A novel method for DNA delivery into bacteria using cationic copolymers. (2021)
- Authors:
- USP affiliated authors: FLORENZANO, FÁBIO HERBST - EEL ; SALINAS, ROBERTO KOPKE - IQ ; CUCCOVIA, IOLANDA MIDEA - IQ ; SOUZA, VALDOMIRO VAGNER DE - IQ ; VITALE, PHELIPE AUGUSTO MARIANO - IQ
- Unidades: EEL; IQ
- DOI: 10.1590/1414-431X202010743
- Subjects: DNA; CÉLULAS EUCARIÓTICAS; GENÉTICA BACTERIANA
- Agências de fomento:
- Language: Inglês
- Abstract: Amphiphilic copolymers have a wide variety of medical and biotechnological applications, including DNA transfection in eukaryotic cells. Still, no polymer-primed transfection of prokaryotic cells has been described. The reversible addition-fragmentation chain transfer (RAFT) polymer synthesis technique and the reversible deactivation radical polymerization variants allow the design of polymers with well-controlled molar mass, morphology, and hydrophilicity/hydrophobicity ratios. RAFT was used to synthesize two amphiphilic copolymers containing different ratios of the amphiphilic poly[2-(dimethyl-amino) ethyl methacrylate] and the hydrophobic poly [methyl methacrylate]. These copolymers bound to pUC-19 DNA and successfully transfected non-competent Escherichia coli DH5α, with transformation efficiency in the range of 103 colony-forming units per µg of plasmid DNA. These results demonstrate prokaryote transformation using polymers with controlled amphiphilic/hydrophobic ratios.
- Imprenta:
- Source:
- Título do periódico: Brazilian Journal of Medical and Biological Research
- ISSN: 1414-431X
- Volume/Número/Paginação/Ano: v. 54, n. 5, 8 p
- Este periódico é de acesso aberto
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: gold
- Licença: cc-by
-
ABNT
SOUZA, V. V et al. A novel method for DNA delivery into bacteria using cationic copolymers. Brazilian Journal of Medical and Biological Research, v. 54, n. 5, 2021Tradução . . Disponível em: https://doi.org/10.1590/1414-431X202010743. Acesso em: 01 maio 2024. -
APA
Souza, V. V., Vitale, P. A. M., Florenzano, F. H., Cuccovia, I. M., & Salinas, R. K. (2021). A novel method for DNA delivery into bacteria using cationic copolymers. Brazilian Journal of Medical and Biological Research, 54( 5). doi:10.1590/1414-431X202010743 -
NLM
Souza VV, Vitale PAM, Florenzano FH, Cuccovia IM, Salinas RK. A novel method for DNA delivery into bacteria using cationic copolymers. [Internet]. Brazilian Journal of Medical and Biological Research. 2021 ; 54( 5):[citado 2024 maio 01 ] Available from: https://doi.org/10.1590/1414-431X202010743 -
Vancouver
Souza VV, Vitale PAM, Florenzano FH, Cuccovia IM, Salinas RK. A novel method for DNA delivery into bacteria using cationic copolymers. [Internet]. Brazilian Journal of Medical and Biological Research. 2021 ; 54( 5):[citado 2024 maio 01 ] Available from: https://doi.org/10.1590/1414-431X202010743 - Nanoparticles of PMMA-b-PDMAEMA for gene delivery - P202
- Complexation studies of polymers with DNA and gene delivery assays into E. coli DH5α
- A novel method for DNA delivery into bacteria using cationic copolymers
- Efeito de pH e composição de fosfolipídicos na interação do copolímero 'PMMA IND. 1'-Ь-'PDMAEMA IND. 6,3' com vesículas
- Caracterização do copolímero PMMA (1) - b - PDMAEMA (6,3) e estudo da permeabilização de vesículas preparadas com fosfolipídios
- Study of the effect of copolymer structure on the permeability of phospholipid vesicles
- Mecanismos de ação do copolímero 'PMMA IND. 1'-Ь-'PDMAEMA IND. 3' em modelos de membrana com diferentes cargas
- Caracterização do copolímero PMMA(1)-b-PDMAEMA(1,3) e estudo do seu efeito sobre a permeabilidade de vesículas de fosfolipídios
- Explorando padrões estruturais de polímeros na interação com vesículas de fosfolipidios
- Characterization of PMMA-b-PDMAEMA aggregates in aqueous solutions
Informações sobre o DOI: 10.1590/1414-431X202010743 (Fonte: oaDOI API)
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