Artigo de periodico

Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction. (2021)

• Authors:
  • Torres-Silva, G.
  • Correia, Ludmila Nayara de Freitas
  • Batista, Diego Silva
  • Koehler, Andréa Dias
  • Resende, Sheila Vitória
  • Romanel, Elisson Antonio
  • Cassol, Daniela
  • Almeida, Ana Maria Rocha
  • Strickler, Susan E
  • Specht, Chelsea D.
  • Otoni, Wagner Campos
• Autor USP: ROMANEL, ELISSON ANTÔNIO DA COSTA - EEL
• Unidade: EEL
• DOI: 10.3389/fpls.2021.697556
• Subjects: CALMODULINA; SEQUENCIAMENTO GENÉTICO; RNA
• Agências de fomento:
  • Financiamento CNPq
  • Financiamento FAPEMIG
  • Financiamento CAPES
• Language: Inglês
• Abstract: Melocactus glaucescens is an endangered cactus highly valued for its ornamental properties. In vitro shoot production of this species provides a sustainable alternative to overharvesting from the wild; however, its propagation could be improved if the genetic regulation underlying its developmental processes were known. The present study generated de novo transcriptome data, describing in vitro shoot organogenesis induction in M. glaucescens. Total RNA was extracted from explants before (control) and after shoot organogenesis induction (treated). A total of 14,478 unigenes (average length, 520 bases) were obtained using Illumina HiSeq 3000 (Illumina Inc., San Diego, CA, USA) sequencing and transcriptome assembly. Filtering for differential expression yielded 2,058 unigenes. Pairwise comparison of treated vs. control genes revealed that 1,241 (60.3%) unigenes exhibited no significant change, 226 (11%) were downregulated, and 591 (28.7%) were upregulated. Based on database analysis, more transcription factor families and unigenes appeared to be upregulated in the treated samples than in controls. Expression of WOUND INDUCED DEDIFFERENTIATION 1 (WIND1) and CALMODULIN (CaM) genes, both of which were upregulated in treated samples, was further validated by real-time quantitative PCR (RT-qPCR). Differences in gene expression patterns between control and treated samples indicate substantial changes in the primary and secondary metabolism of M. glaucescens after the induction of shoot organogenesis. These results help to clarify the molecular genetics and functional genomic aspects underlying propagation in the Cactaceae family.
• Source:
  • Título do periódico: Frontiers in Plant Science
  • ISSN: 1664-462X
  • Volume/Número/Paginação/Ano: v. 12, p. 1-15, 2021

Informações sobre o DOI: 10.3389/fpls.2021.697556 (Fonte: oaDOI API)
• Este periódico é de acesso aberto
• Este artigo é de acesso aberto
• URL de acesso aberto
• Cor do Acesso Aberto: gold
• Licença: cc-by

---

How to cite

A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas

• ABNT

  TORRES-SILVA, G. et al. Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction. Frontiers in Plant Science, v. 12, p. 1-15, 2021Tradução . . Disponível em: https://doi.org/10.3389/fpls.2021.697556. Acesso em: 21 maio 2024.

• APA

  Torres-Silva, G., Correia, L. N. de F., Batista, D. S., Koehler, A. D., Resende, S. V., Romanel, E. A., et al. (2021). Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction. Frontiers in Plant Science, 12, 1-15. doi:10.3389/fpls.2021.697556

• NLM

  Torres-Silva G, Correia LN de F, Batista DS, Koehler AD, Resende SV, Romanel EA, Cassol D, Almeida AMR, Strickler SE, Specht CD, Otoni WC. Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction. [Internet]. Frontiers in Plant Science. 2021 ; 12 1-15.[citado 2024 maio 21 ] Available from: https://doi.org/10.3389/fpls.2021.697556

• Vancouver

  Torres-Silva G, Correia LN de F, Batista DS, Koehler AD, Resende SV, Romanel EA, Cassol D, Almeida AMR, Strickler SE, Specht CD, Otoni WC. Transcriptome Analysis of Melocactus glaucescens (Cactaceae) Reveals Metabolic Changes During in vitro Shoot Organogenesis Induction. [Internet]. Frontiers in Plant Science. 2021 ; 12 1-15.[citado 2024 maio 21 ] Available from: https://doi.org/10.3389/fpls.2021.697556

Últimas obras dos mesmos autores vinculados com a USP cadastradas na BDPI:

• Análise da expressão de genes BAHD em cana-de-açúcar (Saccharum spontaneum) potencialmente envolvidos na incorporação de ácidos hidroxicinâmicos
• Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)
• Reference gene selection for qRT-PCR in Brazilian-ginseng [Pfaffia glomerata (Spreng.) Pedersen] as affected by various abiotic factors
• Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions.
• The floral transcriptome of Eucalyptus grandis
• Expression of Melocactus glaucescens SERK1 sheds new light on the mechanism of areolar activation in cacti.
• Lignin and cellulose synthesis and antioxidative defense mechanisms are affected by light quality in Brachypodium distachyon
• Complete genome sequences of two new virus isolates associated with cotton blue disease resistance breaking in Brazil
• Analysis of the PEBP gene family and identification of a novel FLOWERING LOCUS T</i> orthologue in sugarcane
• microRNAs as reference genes for quantitative PCR in cotton