Xylan, Xylooligosaccharides, and Aromatic Structures With Antioxidant Activity Released by Xylanase Treatment of Alkaline-Sulfite?Pretreated Sugarcane Bagasse. (2022)
- Authors:
- USP affiliated authors: FERRAZ, ANDRÉ LUIS - EEL ; MILAGRES, ADRIANE MARIA FERREIRA - EEL ; SILVA, VERÔNICA TÁVILLA FERREIRA - EEL ; RUSCHONI, UIRAJÁ CAYOWA MAGALHÃES - EEL
- Unidade: EEL
- DOI: 10.3389/fbioe.2022.940712
- Subjects: BIOTECNOLOGIA; CANA-DE-AÇÚCAR; ANTIOXIDANTES
- Keywords: sugarcane; antioxidants; alkaline-sulfite pretreatment; xylooligosaccharides; xylanase; biorefinery
- Agências de fomento:
- Language: Inglês
- Abstract: Xylanase enzymes are useful to fractionate plant biomass, producing xylan,xylooligosaccharides (XOS), and antioxidant-derived XOS. In a biorefinery, pretreatedbiomass can be digested with xylanase prior to cellulose saccharification, enhancing theproduct portfolio in the process. With this vision, this study highlighted a wide range of newproducts attainable from alkaline-sulfite–pretreated sugarcane bagasse by treatments withendo-xylanase under controlled conditions. The developed process provided a crude extractcorresponding to 29.7% (w/w) of pretreated sugarcane bagasse. The crude extract included arelatively polymeric glucuronoarabinoxylan fraction, DP2-DP6 xylooligosaccharides, andaromatic compounds. The enzymatically produced extract was fractionated with increasingethanol concentrations [up to 90% (v/v)], providing precipitation of varied polymeric xylanfractions (48% (w/w) of the crude extract) with average molar masses ranging from 28 kDa to3.6 kDa. The fraction soluble in 90% ethanol was subjected to adsorption on 4% (w/v)activated charcoal and eluted with an ethanol gradient from 10% to 70% (v/v), thus providingxylooligosaccharides and aromatic fractions. Most of the xylooligosaccharides (74% of theeluted sugars) were washed out in 10%–30% ethanol. DP2 and DP3 structures predominatedin the 10% ethanol fraction, while DP5 structures were significantly enriched in the 30% ethanolfraction. Higher ethanol concentrations desorbed xylooligosaccharides associated with higheramounts of aromatic compounds. Total aromatics, phenolic structures, andp-hydroxycinnamates predominated in the fractions desorbed with 60% and 70% ethanol.The antioxidant activity of produced fractions correlated with their phenolic contents. Compiledresults indicate that a wide variety of products can be prepared from pretreated biomass usingxylanase-aided extraction procedures. Recovered fractions presented different
- Imprenta:
- Source:
- Título do periódico: Frontiers in bioengineering and biotechnology
- ISSN: 2296-4185
- Volume/Número/Paginação/Ano: v.10, n. 940712, p.1-10, 2022
- Este periódico é de acesso aberto
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: gold
- Licença: cc-by
-
ABNT
SILVA, Veronica Távilla F. et al. Xylan, Xylooligosaccharides, and Aromatic Structures With Antioxidant Activity Released by Xylanase Treatment of Alkaline-Sulfite?Pretreated Sugarcane Bagasse. Frontiers in bioengineering and biotechnology, v. 10, n. 940712, p. 1-10, 2022Tradução . . Disponível em: https://doi.org/10.3389/fbioe.2022.940712. Acesso em: 28 abr. 2024. -
APA
Silva, V. T. F., Ruschoni, U. C. M., Ferraz, A. L., & Milagres , A. M. F. (2022). Xylan, Xylooligosaccharides, and Aromatic Structures With Antioxidant Activity Released by Xylanase Treatment of Alkaline-Sulfite?Pretreated Sugarcane Bagasse. Frontiers in bioengineering and biotechnology, 10( 940712), 1-10. doi:10.3389/fbioe.2022.940712 -
NLM
Silva VTF, Ruschoni UCM, Ferraz AL, Milagres AMF. Xylan, Xylooligosaccharides, and Aromatic Structures With Antioxidant Activity Released by Xylanase Treatment of Alkaline-Sulfite?Pretreated Sugarcane Bagasse. [Internet]. Frontiers in bioengineering and biotechnology. 2022 ;10( 940712): 1-10.[citado 2024 abr. 28 ] Available from: https://doi.org/10.3389/fbioe.2022.940712 -
Vancouver
Silva VTF, Ruschoni UCM, Ferraz AL, Milagres AMF. Xylan, Xylooligosaccharides, and Aromatic Structures With Antioxidant Activity Released by Xylanase Treatment of Alkaline-Sulfite?Pretreated Sugarcane Bagasse. [Internet]. Frontiers in bioengineering and biotechnology. 2022 ;10( 940712): 1-10.[citado 2024 abr. 28 ] Available from: https://doi.org/10.3389/fbioe.2022.940712 - In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse
- Anatomic and ultrastructural characteristics of different regions of sugar cane internodes which affect their response to alkaline-sulfite pretreatment and material recalcitrance
- Sugarcane hybrids with original low lignin contents and high field productivity are useful to reach high glucose yields from bagasse
- Topochemical characterization of sugar cane pretreated with alkaline sulfite
- Topochemical characterization of sugar cane pretreated with alkaline sulfite
- Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin
- An innovative concept for industrial sugarcane processing enhances polysaccharide utilization in first- and second-generation integrated biorefineries
- Chemical modification in xylan/hydroxycinnamic acids mediated by laccases
- The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures
- Techno-economic impacts of varied compositional profiles of sugarcane experimental hybrids on a biorefinery producing sugar, ethanol and electricity
Informações sobre o DOI: 10.3389/fbioe.2022.940712 (Fonte: oaDOI API)
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