Integrated bioinformatics, modelling, and gene expression analysis of the putative pentose transporter from Candida tropicalis during xylose fermentation with and without glucose addition (2022)
- Authors:
- USP affiliated authors: FELIPE, MARIA DAS GRAÇAS DE ALMEIDA - EEL ; SEGATO, FERNANDO - EEL ; SILVA, TATIANE DA FRANCA - EEL ; OLIVA, BIANCA - EEL ; QUEIROZ, SARAH DE SOUZA - EEL
- Unidade: EEL
- DOI: 10.1007/s00253-022-12005-x
- Subjects: TRANSPORTE EPITELIAL; CÉLULAS EPITELIAIS; GENÉTICA; BIOLOGIA; BIOLOGIA CELULAR; CÉLULAS
- Keywords: Xylose; Glucose; Transport; Candida tropicalis
- Agências de fomento:
- Language: Inglês
- Abstract: The transport of substrates across the cell membrane plays an essential role in nutrient assimilation by yeasts. The establishment of an efficient microbial cell factory, based on the maximum use of available carbon sources, can generate new technologies that allow the full use of lignocellulosic constituents. These technologies are of interest because they could promote the formation of added-value products with economic feasibility. In silico analyses were performed to investigate gene sequences capable of encoding xylose transporter proteins in the Candida tropicalis genome. The current study identified 11 putative transport proteins that have not yet been functionally characterized. A phylogenetic tree highlighted the potential C. tropicalis xylose-transporter proteins CtXUT1, CtXUT4, CtSTL1, CtSTL2, and CtGXT2, which were homologous to previously characterized and reported xylose transporters. Their expression was quantified through real-time qPCR at defined times, determined through a kinetic analysis of the microbial growth curve in the absence/presence of glucose supplemented with xylose as the main carbon source. The results indicated different mRNA expression levels for each gene. CtXUT1 mRNA expression was only found in the absence of glucose in the medium. Maximum CtXUT1 expression was observed in intervals of the highest xylose consumption (21 to 36 h) that corresponded to consumption rates of 1.02 and 0.82 g/L/h in the formulated media, with xylose as the only carbon source and with glucose addition. These observations indicate that CtXUT1 is an important xylose transporter in C. tropicalis.
- Imprenta:
- Source:
- Título do periódico: Applied microbiology and biotechnology
- ISSN: 0175-7598
- Volume/Número/Paginação/Ano: v.106, p.4587-4606, 2022
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
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ABNT
QUEIROZ, Sarah de Souza et al. Integrated bioinformatics, modelling, and gene expression analysis of the putative pentose transporter from Candida tropicalis during xylose fermentation with and without glucose addition. Applied microbiology and biotechnology, v. 106, p. 4587-4606, 2022Tradução . . Disponível em: https://doi.org/10.1007/s00253-022-12005-x. Acesso em: 28 abr. 2024. -
APA
Queiroz, S. de S., Oliva, B., Silva, T. F., Segato, F., & Felipe, M. das G. de A. (2022). Integrated bioinformatics, modelling, and gene expression analysis of the putative pentose transporter from Candida tropicalis during xylose fermentation with and without glucose addition. Applied microbiology and biotechnology, 106, 4587-4606. doi:10.1007/s00253-022-12005-x -
NLM
Queiroz S de S, Oliva B, Silva TF, Segato F, Felipe M das G de A. Integrated bioinformatics, modelling, and gene expression analysis of the putative pentose transporter from Candida tropicalis during xylose fermentation with and without glucose addition [Internet]. Applied microbiology and biotechnology. 2022 ;106 4587-4606.[citado 2024 abr. 28 ] Available from: https://doi.org/10.1007/s00253-022-12005-x -
Vancouver
Queiroz S de S, Oliva B, Silva TF, Segato F, Felipe M das G de A. Integrated bioinformatics, modelling, and gene expression analysis of the putative pentose transporter from Candida tropicalis during xylose fermentation with and without glucose addition [Internet]. Applied microbiology and biotechnology. 2022 ;106 4587-4606.[citado 2024 abr. 28 ] Available from: https://doi.org/10.1007/s00253-022-12005-x - Processos de produção de ácido celobiônico (cba) a partir de material de celulose ou lignocelulose
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Informações sobre o DOI: 10.1007/s00253-022-12005-x (Fonte: oaDOI API)
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