Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate (2023)
- Authors:
- USP affiliated authors: AGUIAR, LEANDRO GONÇALVES DE - EEL ; FREITAS, LARISSA DE - EEL ; RANGEL, AMANDA BAHIA DE SOUZA - EEL ; SILVA, MATEUS VINICIUS CASAGRANDE DA - EEL
- Unidade: EEL
- DOI: 10.1007/s00449-023-02928-8
- Assunto: BIOTECNOLOGIA
- Keywords: Computer simulation; Esterifcation; Immobilized lipase; Magnetic polymer support; Magnetically stabilized fuidized bed bioreactor
- Agências de fomento:
- Language: Inglês
- Abstract: This study aimed to develop and investigate the synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on magnetic poly(styrene-co-divinylbenzene) (STY–DVB-M) particles in a magnetically stabilized fuidized bed reactor (MSFBR) operated in continuous mode. The physical properties of the copolymer were characterized by Fouriertransform infrared spectroscopy (FTIR), diferential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The glass transition temperature was 85.68 °C, and the onset of thermal degradation occurred at 406.66 °C. Syntheses were performed at 50 °C using a space time of 12 h and a bed porosity of 0.892. Assays were conducted to assess the infuence of magnetic feld intensity (5 to 15 mT) on reaction yield, ester concentration, and productivity. The highest productivity was 0.850±0.023 mmol g−1 h−1, obtained with a magnetic feld intensity of 15 mT. An operational stability test was performed under these conditions, revealing a biocatalyst half-life of 2148 h (179 operation cycles) and a thermal deactivation constant of 3.23× 10−4 h−1 (R2=0.9446). Computational simulations and mathematical modeling were performed using Scilab based on ping-pong bi–bi kinetics and molar balances of reaction species. The model provided consistent results of interstitial velocity and good prediction of reaction yields, with R2=0.926. These fndings demonstrate that the studied technique can provide improvements in biocatalytic processes, representing a promising strategy for the enzymatic synthesis of 2-ethylhexyl oleate.
- Imprenta:
- Publisher: Springer
- Publisher place: Garching, Gw
- Date published: 2023
- Source:
- Título do periódico: Bioprocess and biosystems engineering (internet)
- ISSN: 1615-7605
- Volume/Número/Paginação/Ano: v.46, p.1665-1676, 2023
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
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ABNT
SILVA, Mateus Vinicius Casagrande da et al. Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate. Bioprocess and biosystems engineering (internet), v. 46, p. 1665-1676, 2023Tradução . . Disponível em: https://doi.org/10.1007/s00449-023-02928-8. Acesso em: 03 maio 2024. -
APA
Silva, M. V. C. da, Rangel, A. B. de S., Rosa, C. M. R., Assis, G. P. de, Aguiar, L. G. de, & Freitas, L. (2023). Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate. Bioprocess and biosystems engineering (internet), 46, 1665-1676. doi:10.1007/s00449-023-02928-8 -
NLM
Silva MVC da, Rangel AB de S, Rosa CMR, Assis GP de, Aguiar LG de, Freitas L. Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate [Internet]. Bioprocess and biosystems engineering (internet). 2023 ;46 1665-1676.[citado 2024 maio 03 ] Available from: https://doi.org/10.1007/s00449-023-02928-8 -
Vancouver
Silva MVC da, Rangel AB de S, Rosa CMR, Assis GP de, Aguiar LG de, Freitas L. Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate [Internet]. Bioprocess and biosystems engineering (internet). 2023 ;46 1665-1676.[citado 2024 maio 03 ] Available from: https://doi.org/10.1007/s00449-023-02928-8 - Estudo cinético e termodinâmico da síntese do oleato de 2-etilhexila catalisada por lipase B de Candida antarctica imobilizada em copolímeros magnéticos
- Obtention of a mathematical model for ester synthesis mediated by a commercial lipase immobilized on a magnetized copolymer
- Synthesis and Characterization of Magnetic Poly(STY-EGDMA) Particles for Application as Biocatalyst Support in Octyl Oleate Ester Synthesis: Kinetic and Thermodynamic Parameters and Mathematical Modeling
- Kinetic and Thermodynamic Study of 2-Ethylhexyl Oleate Synthesis Catalyzed by Candida antarctica Lipase Immobilized on a Magnetic Hybrid Support
- Kinetic study of isopropyl palmitate synthesis catalyzed by lipases immobilized on a magnetic copolymer support
- Kinetic study of isopropyl palmitate synthesis catalyzed by lipases immobilized on a magnetic copolymer support
- Synthesis of octyl oleate catalyzed by lipase immobilized on magnetized copolymer
- Synthesis of isopropyl palmitate by lipase immobilized on magnetized polymer matrix
- Synthesis of 2-ethylhexyl oleate catalyzed by lipase immobilized on a magnetic support in packed-bed bioreactor
- Síntese do palmitato de isopropila catalisada por lipase imobilizada em copolímero magnetizado
Informações sobre o DOI: 10.1007/s00449-023-02928-8 (Fonte: oaDOI API)
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