In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse (2019)
- Authors:
- USP affiliated authors: MILAGRES, ADRIANE MARIA FERREIRA - EEL ; FERRAZ, ANDRÉ LUIS - EEL
- Unidade: EEL
- Assunto: BIOTECNOLOGIA
- Keywords: Sugarcane bagasse; Bacillus pumilus; Xylanase; Xylan extraction; Xylooligosaccharides; Luminase
- Language: Inglês
- Abstract: The purpose of this work was to investigate the enzymatic extraction of xylan using xylanases active in alkaline conditions. One on-site produced and three commercial xylanases designed to boost bleaching of pulped wood were characterized for their optimal pH, temperature and stability to compare their effects on xylan extraction from pretreated sugarcane bagasse. On-site produced xylanase was prepared from cultures of the alkaliphilic Bacillus pumilus CBMAI 0008 strain. The optimal temperatures and pH levels for all commercial xylanases were 60 °C and 6, whereas for B. pumilus xylanase, they were 50 °C and 8, respectively. B. pumilus was active up to pH 10, but it had low thermal stability. Five sugarcane substrates were prepared to contain decreasing lignin content based on alkaline-sulfite pretreatment or acid-chlorite delignification. Xylanase doses (5–100 IU/g substrate) were applied to these substrates at pH 8.0, 50 °C and a 24-h reaction. Xylan extraction yields were strongly dependent on the lignin content of the substrates. Maximal xylan extraction yields were obtained in acid-chlorite delignified substrates, reaching values of 64% and 45% for Luminase and B. pumilus xylanases, respectively. Residual (non-extractable) xylans seemed occluded by and/or complexed with residual lignin of the substrates.
- Imprenta:
- Source:
- Título do periódico: EUBCE 2019 27th European Biomass Conference & Exhibition
- Volume/Número/Paginação/Ano: 2019
-
ABNT
SANTOS, Maiara Pedral dos et al. In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse. 2019, Anais.. Lisboa: ETA, 2019. . Acesso em: 02 maio 2024. -
APA
Santos, M. P. dos, Távilla, V., Reinoso, F. A. M., Ferraz, A. L., & Milagres , A. M. F. (2019). In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse. In EUBCE 2019 27th European Biomass Conference & Exhibition. Lisboa: ETA. -
NLM
Santos MP dos, Távilla V, Reinoso FAM, Ferraz AL, Milagres AMF. In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse. EUBCE 2019 27th European Biomass Conference & Exhibition. 2019 ;[citado 2024 maio 02 ] -
Vancouver
Santos MP dos, Távilla V, Reinoso FAM, Ferraz AL, Milagres AMF. In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse. EUBCE 2019 27th European Biomass Conference & Exhibition. 2019 ;[citado 2024 maio 02 ] - Anatomic and ultrastructural characteristics of different regions of sugar cane internodes which affect their response to alkaline-sulfite pretreatment and material recalcitrance
- Sugarcane hybrids with original low lignin contents and high field productivity are useful to reach high glucose yields from bagasse
- Topochemical characterization of sugar cane pretreated with alkaline sulfite
- Topochemical characterization of sugar cane pretreated with alkaline sulfite
- Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin
- An innovative concept for industrial sugarcane processing enhances polysaccharide utilization in first- and second-generation integrated biorefineries
- Techno-economic impacts of varied compositional profiles of sugarcane experimental hybrids on a biorefinery producing sugar, ethanol and electricity
- The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures
- Xylan, Xylooligosaccharides, and Aromatic Structures With Antioxidant Activity Released by Xylanase Treatment of Alkaline-Sulfite?Pretreated Sugarcane Bagasse.
- On-site produced and commercially available alkali-active xylanases compared for xylan extraction from sugarcane bagasse
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