Transport and retention of coliphage T4 in soil columns from a cemetery in São Paulo, Brazil (2001)
- Authors:
- USP affiliated authors: PELLIZARI, VIVIAN HELENA - ICB ; PACHECO, ALBERTO - IGC
- Unidades: ICB; IGC
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Inadequate site and operation of cemeteries in urban areas may contaminate the water resources by microorganisms that grow during the process of corpse decomposition or by pathogenic organisms that may exist in the corpses. If the unconfined aquifer is contaminated in the internal area of the cemetery, that contamination may flow to surrounding areas and cause a health hazard to the people that use that water. Investigation studies at Cemetery Vila Nova Cachoeirinha, São Paulo, Brazil, have shown that the unconfined aquifer is contaminated by microorganisms. Columns under conditions of saturation and continuous flow were used to simulate virus migration in aquifers. Soil columns were prepared by carefully packing dry soil to the desired depth in 40 cm pyrex cylinder. Two columns were prepared, the first one was built up with high sand content samples and the other with high clay content samples. The coliphage T4 (ATCC: 11303-B4, host: Escherichia coli B) was used to simulate virus transport through the soil. The bacteriophage T4 was highly adsorbed to samples with high clay content, while adsorption to soils with high sand content was low. Biological (T4) and chemical (NaCl - 1%) tracers were injected into the column and the effluent was monitored for their presence. Relative concentration (C/Co) versus time curves of the effluent were plotted to study the retention of the coliphage by in the sand and clay columns. The high clay content columns retained morecoliphage than the sand columns. Moreover, the coliphage took more time to be transported through the high clay content column. This study shows that coliphage T4 has been successfully used to simulate virus transport through soils
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB-USP
- Publisher place: São Paulo
- Date published: 2001
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
-
ABNT
RODRIGUES, D. F. et al. Transport and retention of coliphage T4 in soil columns from a cemetery in São Paulo, Brazil. 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB-USP, 2001. Disponível em: https://repositorio.usp.br/directbitstream/e4b2059c-8f7f-4b63-b116-a8f0fe2a6d7c/1190359.pdf. Acesso em: 26 abr. 2024. -
APA
Rodrigues, D. F., Matos, B. A., Gamba, R., Pacheco, A., & Pellizari, V. H. (2001). Transport and retention of coliphage T4 in soil columns from a cemetery in São Paulo, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB-USP. Recuperado de https://repositorio.usp.br/directbitstream/e4b2059c-8f7f-4b63-b116-a8f0fe2a6d7c/1190359.pdf -
NLM
Rodrigues DF, Matos BA, Gamba R, Pacheco A, Pellizari VH. Transport and retention of coliphage T4 in soil columns from a cemetery in São Paulo, Brazil [Internet]. Resumos. 2001 ;[citado 2024 abr. 26 ] Available from: https://repositorio.usp.br/directbitstream/e4b2059c-8f7f-4b63-b116-a8f0fe2a6d7c/1190359.pdf -
Vancouver
Rodrigues DF, Matos BA, Gamba R, Pacheco A, Pellizari VH. Transport and retention of coliphage T4 in soil columns from a cemetery in São Paulo, Brazil [Internet]. Resumos. 2001 ;[citado 2024 abr. 26 ] Available from: https://repositorio.usp.br/directbitstream/e4b2059c-8f7f-4b63-b116-a8f0fe2a6d7c/1190359.pdf - Detection of Cryptosporidium oocysts in groundwater utilized for consumption in Itaquaquecetuba, S.Paulo-Brazil
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