UV light-induced apoptosis is dependent of damaged DNA replication in mammalian cells (2003)
- Autores:
- Autor USP: MENCK, CARLOS FREDERICO MARTINS - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Idioma: Português
- Resumo: Ultraviolet light (UV) induces helix-distorting lesions in DNA (Friedberg, 1995). Several studies indicate that one of this lesions, cyclobutane pyrimidine dimers (CPDs), are involved in signaling for UV-induced apoptosis in mammalian cells (Chiganças et al, 2002). However, the role of the damaged DNA replication in this process is not quite clear. To investigate the importance of injured DNA replication in UV-induced apoptosis we utilized aphidicolin, an inhibitor of DNA replication (Spadari, 1984) in wild type and XPB mutated Chinese hamster ovary (CHO) cells expressing a marsupial photolyase (phr gene from Potorous tridactylus) that removes specifically CPDs from the damaged DNA. Our results not only confirmed the efficiency of aphidicolin in blocking DNA synthesis, but also indicated that UV light (UV-C, 254 nm) alone inhibits DNA replication in the hours immediatly subsequent to cells irradiation.In experiments with flow citometry (Fluorescense Activated Cell Sorter-FACS), we were able to quantify the apoptosis ratios in irradiated cells. In these experiments, we demonstrated that cells that had their DNA synthesis blocked after UV are prevented of entering into apoptosis. This result was observed in both wild type and XPB mutated cells, which indicates that the protection observed is independent of NER (nucleotide excision repair). This fact indicates that apoptosis upon UV irradiation requires DNA replicationindependently of the cell proficiency to repair the damaged double-helix
- Imprenta:
- Editora: Comissão de Pesquisa do ICB/USP
- Local: São Paulo
- Data de publicação: 2003
- Fonte:
- Título do periódico: Resumos
- Nome do evento: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
BATISTA, Luís Francisco Zirnberger e MENCK, Carlos Frederico Martins e CHIGANÇAS, Vanessa. UV light-induced apoptosis is dependent of damaged DNA replication in mammalian cells. 2003, Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. . Acesso em: 19 abr. 2024. -
APA
Batista, L. F. Z., Menck, C. F. M., & Chiganças, V. (2003). UV light-induced apoptosis is dependent of damaged DNA replication in mammalian cells. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP. -
NLM
Batista LFZ, Menck CFM, Chiganças V. UV light-induced apoptosis is dependent of damaged DNA replication in mammalian cells. Resumos. 2003 ;[citado 2024 abr. 19 ] -
Vancouver
Batista LFZ, Menck CFM, Chiganças V. UV light-induced apoptosis is dependent of damaged DNA replication in mammalian cells. Resumos. 2003 ;[citado 2024 abr. 19 ] - Restoring DNA repair capacity of cells from three distinct diseases by XPD gene-recombinant adenovirus
- DNA-Based biological dosimeters for the assessment of photo protection of sunscreens
- DNA repair and consequences of DNA damage: in the fight against and causing cancer!
- ATR DNA damage response after UVB in polimerase ETA deficient cells
- Construção de um adenovírus recombinante portando o cDNA do gene PHR2 de cana-de-açúcar
- XPD transduction in human cells deficient in DNA repair by recombinant adenovirus
- Construction of an adenoviral vector carrying the cDNA of XPD gene for complementation of human cells deficient in dna repair
- Estudos da função da proteína THI1 através de ensaios bioquímicos
- Introdução do gene de fotoliase em células humanas
- Genetic systems for the hepatitis B virus surface gene expression
Como citar
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
