Clonagem da região promotora da protease FtsH - p1 de Arabidopsis thaliana (2003)
- Autores:
- Autor USP: SILVA FILHO, MARCIO DE CASTRO - ESALQ
- Unidade: ESALQ
- Assuntos: CLONAGEM; MELHORAMENTO GENÉTICO VEGETAL
- Idioma: Português
- Resumo: The eukaryotic FtsH proteases falls in five phylogenetic related groups composed exclusively of mitochondria (FtsH-m1 and FtsH-m2) or plastid proteins (FtsH-p1, FtsH-p2 and FtsH-p3) that are involved in crucial cellular processes as oxidative phosphorylation, photosynthesis, and biogenesis of mitochondria and plastid. Despite the central role of eukaryotic FtsH proteases in several cellular processes, the regulation of the expression of these proteins, as well as its function in plants, is poorly known. We aimed in this work to clone the putative promoter region from FtsH-p1 gene from Arabidopsis thaliana. Once the complete genome sequence of Arabidopsis is available on the WEB (http://www.ncbi.nlm.nih.gov/), the promoter region of virtually all its genes could be amplified by PCR (Polimerase Chain Reaction). Then, using this approach, we designed primers based on available genome sequence and amplified by PCR the intergenic region upstream to initiation codon of gene FtsH-p1. Later this region was cloned in the PstI/NcoI sites of the binary vector pCAMBIA-1281z, driving the expression of the gene reporter uid A. The promoter region of the FtsH-p1 gene was successfully amplified by PCR and cloned into the binary vector pCAMBIA-1281Z controlling de expression of the gene reporter uid A showing the viability of this strategy
- Imprenta:
- Fonte:
- Título do periódico: Agropecuária; resumos
- Nome do evento: Simposio Internacional de Iniciação Cientifica da Universidade de São Paulo
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ABNT
FIORI, C. S. et al. Clonagem da região promotora da protease FtsH - p1 de Arabidopsis thaliana. 2003, Anais.. São Paulo: USP, 2003. . Acesso em: 23 abr. 2024. -
APA
Fiori, C. S., Nunes, S. D. V., Marbach, P. A. S., & Silva Filho, M. de C. (2003). Clonagem da região promotora da protease FtsH - p1 de Arabidopsis thaliana. In Agropecuária; resumos. São Paulo: USP. -
NLM
Fiori CS, Nunes SDV, Marbach PAS, Silva Filho M de C. Clonagem da região promotora da protease FtsH - p1 de Arabidopsis thaliana. Agropecuária; resumos. 2003 ;[citado 2024 abr. 23 ] -
Vancouver
Fiori CS, Nunes SDV, Marbach PAS, Silva Filho M de C. Clonagem da região promotora da protease FtsH - p1 de Arabidopsis thaliana. Agropecuária; resumos. 2003 ;[citado 2024 abr. 23 ] - Evolutionary history of Arabidopsis thaliana aminoacyl-tRNA synthetase dual targeted proteins
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