Effect of TNF-alfa the mouse preimplantation embryo development in vitro (2004)
- Authors:
- USP affiliated authors: BEVILACQUA, ESTELA MARIS ANDRADE FORELL - ICB ; BELIZARIO, JOSE ERNESTO - ICB
- Unidade: ICB
- Subjects: HISTOLOGIA; FARMACOLOGIA
- Language: Inglês
- Abstract: During the development, embryos are under the effects of different cytokines, growth factors and other regulatory molecules present in the female tract. Particularly interesting is the presence of TNF-? at physiological doses in these sites and, at high concentrations in inflammatory processes. Studies focusing the embryonic susceptibility to the TNF-? have been done in different species, but most of these were directed to the blastocyst stage. In this study, mice embryos were collected at 4-8 cells phase, cultivated for 24 h in the presence or absence of different concentrations of TNF-? and, morphologically analyzed. In the presence of TNF-? the embryos had less blastomeres, modified nuclear morphology and most of them were blocked in the morula phase when compared with the control group. Great part of the TNF-?-treated blocked embryos was positive for caspase-8 and reactive for anexina-V indicating the entrance in apoptosis process. Similar but less frequent responses were also found in the control group. The use of impermeable fluorescent dyes and Hoechst was also important for characterizing the nuclear integrity of plasma membrane and nuclear morphological alterations in the different experimental groups. These findings suggest that TNF-? may act on the cleavage phase by blocking the course of embryo development, leading to cellular death by apoptosis, adding new information on the embryos susceptibility in the phase of pre-implantation
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
-
ABNT
WOLFF, Philip e BELIZÁRIO, José Ernesto e BEVILACQUA, Estela Maris Andrade Forell. Effect of TNF-alfa the mouse preimplantation embryo development in vitro. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 24 abr. 2024. -
APA
Wolff, P., Belizário, J. E., & Bevilacqua, E. M. A. F. (2004). Effect of TNF-alfa the mouse preimplantation embryo development in vitro. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Wolff P, Belizário JE, Bevilacqua EMAF. Effect of TNF-alfa the mouse preimplantation embryo development in vitro. Abstracts. 2004 ;[citado 2024 abr. 24 ] -
Vancouver
Wolff P, Belizário JE, Bevilacqua EMAF. Effect of TNF-alfa the mouse preimplantation embryo development in vitro. Abstracts. 2004 ;[citado 2024 abr. 24 ] - Cell death and caspase activity in mouse embryos treated with TNF-'alfa'
- Development of bovine and mouse embryos: morphological characteristics
- Estudo comparativo dos aspectos bioquímicops e morfológicos do ciclo celular e apoptose após a fecundação em oócitos de camundongos e de bovinos
- Análise da expressão do gene humano PIF/DCD em camundongos transgênicos
- Proteína emagrece e causa volta do câncer
- Increase in TNF(alpha)-induced apoptotic cell death in hela cells by lovastatin and cyclohexamide is associated with activation of caspase-2, -3, -6, -8
- TNF(alpha)-induced apoptotic cell death in the mouse cell line WEHI-164 correlates with activation of caspase-2, -3, -6, -8, -9
- Mouting evidences of polarized rupture of the outer mitochondrial membrane in apoptotic
- Molecular cloning of new splice variant of human proteolysis-inducing factor (PIF-SV)
- Neurosoft: uma aula prática computacional de drogas que atuam no sistema nervoso central
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
