Macrophage phagocytosis of candida albicans: an in vitro study

Macrophage phagocytosis of candida albicans: an in vitro study (1999)

Authors:
Autor USP: BIRMAN, ESTHER GOLDENBERG - FO
Unidade: FO
DOI: 10.1590/s0103-06631999000300005
Subjects: MICROBIOLOGIA ORAL; PATOLOGIA BUCAL; CANDIDA ALBICANS; ANTIFÚNGICOS
Language: Inglês
Abstract: Considering the role of macrophages in relation to fungi and the various utilized methodologies, the authors established an in vitro model to evaluate macrophage phagocytosis of Candida albicans. Activated macrophages were obtained from the peritoneal cavity of isogenic mice (A/Sn). Two different different strains of Candida albicans serotype A and serotype B with different levels of pathogenicity in vivo and other similar characteristics were utilized in the study. Several microscopic fields contaning about 200 macrophages were counted. The percentage of macrophages phagocytizing at least one viable or nonviable yeast cell determinied an average number of phagocytized yeasts. Neutral red an fluorescein diacetate plus ethidium bromide were used for staining. It is possible to conclude that this is an efficient model related to the used methodology. The average number of yeasts in both strains were similar when inside macrophages, and there was a higher percentage of C. albicans serotype A phagocytosis, wich a was not experimentally pathogenic in vivo
Imprenta:
- Publisher place: São Paulo
- Date published: 1999
Source:
- Título do periódico: Revista de Odontologia da Universidade de São Paulo
- Volume/Número/Paginação/Ano: v. 13, n. 3, p. 233-238, jul./set. 1999

Informações sobre o DOI: 10.1590/s0103-06631999000300005 (Fonte: oaDOI API)

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ABNT

WINFELD, Ilan e BIRMAN, Esther Goldenberg e PAULA, Claudete Rodrigues. Macrophage phagocytosis of candida albicans: an in vitro study. Revista de Odontologia da Universidade de São Paulo, v. 13, n. 3, p. 233-238, 1999Tradução . . Disponível em: https://doi.org/10.1590/s0103-06631999000300005. Acesso em: 23 abr. 2024.
APA

Winfeld, I., Birman, E. G., & Paula, C. R. (1999). Macrophage phagocytosis of candida albicans: an in vitro study. Revista de Odontologia da Universidade de São Paulo, 13( 3), 233-238. doi:10.1590/s0103-06631999000300005
NLM

Winfeld I, Birman EG, Paula CR. Macrophage phagocytosis of candida albicans: an in vitro study [Internet]. Revista de Odontologia da Universidade de São Paulo. 1999 ; 13( 3): 233-238.[citado 2024 abr. 23 ] Available from: https://doi.org/10.1590/s0103-06631999000300005
Vancouver

Winfeld I, Birman EG, Paula CR. Macrophage phagocytosis of candida albicans: an in vitro study [Internet]. Revista de Odontologia da Universidade de São Paulo. 1999 ; 13( 3): 233-238.[citado 2024 abr. 23 ] Available from: https://doi.org/10.1590/s0103-06631999000300005