Miniprep DNA isolation from unicellular and filamentous cyanobacteria (2000)
- Authors:
- USP affiliated authors: FIORE, MARLI DE FATIMA - CENA ; MUI, TSAI SIU - CENA
- Unidade: CENA
- DOI: 10.1016/s0167-7012(99)00110-4
- Assunto: BIOLOGIA MOLECULAR
- Language: Inglês
- Abstract: A rapid miniprep method for isolation of DNA from 12 strains of cyanobacteria belonging to groups I, III, IV and V is described. The protocol is a modification of the methods of Boyle and Lew [Boyle, J.S., Lew, A.M., 1995. An inexpensive alternative to glassmilk for DNA purification. Trends Genet. 11, 8] and the cetyltrimethyl ammonium bromide (CTAB) extraction method of Sahgai-Maroof et al. [Sahgai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W., 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci. USA 81, 8014-8018]. The new method is especially useful for obtaining cyanobacterial DNA from unicellular, filamentous and filamentous branched species. The method does not require phenol extraction and the product can be used directly for PCR amplification and restriction digestion
- Imprenta:
- Source:
- Título do periódico: Journal of Microbiological Methods
- Volume/Número/Paginação/Ano: v. 39, p. 159-159, 2000
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
FIORE, Marli de Fatima et al. Miniprep DNA isolation from unicellular and filamentous cyanobacteria. Journal of Microbiological Methods, v. 39, p. 159-159, 2000Tradução . . Disponível em: https://doi.org/10.1016/s0167-7012(99)00110-4. Acesso em: 17 abr. 2024. -
APA
Fiore, M. de F., Moon, D. H., Tsai, S. M., Lee, H., & Trevors, J. T. (2000). Miniprep DNA isolation from unicellular and filamentous cyanobacteria. Journal of Microbiological Methods, 39, 159-159. doi:10.1016/s0167-7012(99)00110-4 -
NLM
Fiore M de F, Moon DH, Tsai SM, Lee H, Trevors JT. Miniprep DNA isolation from unicellular and filamentous cyanobacteria [Internet]. Journal of Microbiological Methods. 2000 ; 39 159-159.[citado 2024 abr. 17 ] Available from: https://doi.org/10.1016/s0167-7012(99)00110-4 -
Vancouver
Fiore M de F, Moon DH, Tsai SM, Lee H, Trevors JT. Miniprep DNA isolation from unicellular and filamentous cyanobacteria [Internet]. Journal of Microbiological Methods. 2000 ; 39 159-159.[citado 2024 abr. 17 ] Available from: https://doi.org/10.1016/s0167-7012(99)00110-4 - Effect of a highly concentrated lipopeptide extract of Bacillus subtilis on fungal and bacterial cells
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Informações sobre o DOI: 10.1016/s0167-7012(99)00110-4 (Fonte: oaDOI API)
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