Characterization of the non-mevalonate pathway intermadiates in Plasmodium falciparum (2002)
- Authors:
- USP affiliated authors: KIMURA, EDNA TERUKO - ICB ; KATZIN, ALEJANDRO MIGUEL - ICB
- Unidade: ICB
- Subjects: HISTOLOGIA; PARASITOLOGIA
- Language: Inglês
- Abstract: In all organisms studied so far, the biosynthesis of isoprenoids such as dolichol, cholesterol and ubiquinones depends on the condensation of the different numbers of isopentenyl diphosphate (IPP) units. In mammals and fungi, this unit, is derived from classical mevalonate pathway. On the other hand, in higher plants, several algae and some eubacteria, the DOXP pathway was described as alternative non-mevalonate pathway for the synthesis of IPP. This pathway, starts with condensation of pyruvate and glyceraldehyde 3-phosphate which yields 1-deoxy-D-xylulose-5-phosphate (DOXP) as a key metabolite. Two genes encoding the enzymes DOXP-synthase and DOXP-reductoisomerase has recently characterized in Plasmodium falciparum by Jomaa and co-workers (Science, 1999, Vol.285, 1573). Because the DOXP pathway is absent in mammals, this biosynthetic way is a possible target for antimalarial drugs. In our laboratory, we identified for HPLC and electrospray ionization-mass spectroscopy the presence of DOXP in extracts of parasites labeled with [U-14C]-Glycose and [1-14C]-acetic acid. The next metabolite of the way, the 2-C-methy-D-erythritol-4-phosphate (MEP), was not detected at moment. Biochemical studies aiming to identify all the metabolites of the DOXP pathway are been developed in our laboratory
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2002
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
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ABNT
CASSERAL, M B D et al. Characterization of the non-mevalonate pathway intermadiates in Plasmodium falciparum. 2002, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. . Acesso em: 24 abr. 2024. -
APA
Casseral, M. B. D., D'Alexandre, F. L., Kimura, E. A., Peres, V. J., & Katzin, A. M. (2002). Characterization of the non-mevalonate pathway intermadiates in Plasmodium falciparum. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Casseral MBD, D'Alexandre FL, Kimura EA, Peres VJ, Katzin AM. Characterization of the non-mevalonate pathway intermadiates in Plasmodium falciparum. Resumos. 2002 ;[citado 2024 abr. 24 ] -
Vancouver
Casseral MBD, D'Alexandre FL, Kimura EA, Peres VJ, Katzin AM. Characterization of the non-mevalonate pathway intermadiates in Plasmodium falciparum. Resumos. 2002 ;[citado 2024 abr. 24 ] - Protein dolichylation in Plasmodium falciparum
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- Proteasome as target of antimalarial drugs
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