Melatonin action upon insulin signaling in rat hypothalamus (2003)
- Authors:
- USP affiliated authors: CIPOLLA NETO, JOSE - ICB ; CARVALHO, CARLA ROBERTA DE OLIVEIRA - ICB
- Unidade: ICB
- Assunto: FISIOLOGIA
- Language: Português
- Abstract: Objetivo: Melatonin is the pineal hormone that acts via a pertussis toxin-sensitive G-protein to inhibit adenylate cyclase, however the intracellular melatonin signaling effects are not completely understood. Melatonin receptors are mainly present in the suprachiasmatic nucleus (SCN) and pars tuberalis in both humans and rats. The SCN directly controls the circadian rhythm in plasma glucose concentrations that are increased during the night-time period in pinealectomized rats. In this study, we investigated whether melatonin is able to induce tyrosine and serine/threonine phosphorylation, as well as activation, of high molecular weight insulin signaling proteins in the rat hypothalamus. Métodos e Resultados: The phosphorylation status of the transition preoptic-hypothalamic zone, following melatonin intracerebroventricular infusion, was assessed after immunoprecipitation and immunoblotting with antiphosphotyrosine antibody followed by subsequent immunoblottings with anti-IRS-1, anti-JAK-2, and anti-IR antibodies.) IR kinase activity was measured with ATP incubation after IR immunoprecipitation. The effect was already maximal at a melatonin concentration of 10-8M and occurred 5 minutes after infusion. These results indicate that melatonin has the ability to induce tyrosine phosphorylation in IRS-1, IR and JAK-2 in this hypothalamic area. Conclusões: Since these proteins are involved in the insulin and leptin signaling pathways in this CNS fraction, our resultsdemonstrate a potential cross-talk among these three hormones, and also represent an additional signaling mechanism stimulated by melatonin in the rat hypothalamus in vivo
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Curitiba, Paraná
- Date published: 2003
- Source:
- Título do periódico: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
-
ABNT
ANHÊ, Gabriel Forato et al. Melatonin action upon insulin signaling in rat hypothalamus. 2003, Anais.. Curitiba, Paraná: Federação de Sociedades de Biologia Experimental, 2003. . Acesso em: 26 abr. 2024. -
APA
Anhê, G. F., Caperuto, L. C., Souza, L. C. de, Velloso, L. A., Cipolla-Neto, J., & Carvalho, C. R. de O. (2003). Melatonin action upon insulin signaling in rat hypothalamus. In Resumos. Curitiba, Paraná: Federação de Sociedades de Biologia Experimental. -
NLM
Anhê GF, Caperuto LC, Souza LC de, Velloso LA, Cipolla-Neto J, Carvalho CR de O. Melatonin action upon insulin signaling in rat hypothalamus. Resumos. 2003 ;[citado 2024 abr. 26 ] -
Vancouver
Anhê GF, Caperuto LC, Souza LC de, Velloso LA, Cipolla-Neto J, Carvalho CR de O. Melatonin action upon insulin signaling in rat hypothalamus. Resumos. 2003 ;[citado 2024 abr. 26 ] - Insulin temporal sensitivity and its signaling pathway in the rat pineal gland
- Melatonin induces tyrosine phosphorylation of IRS-1 in the rat pre-optic hypothalamic area
- Leptin modulates norepinephrine-mediated melatonin synthesis in cultured rat pineal gland
- Melatonin induces tyrosine phosphorylation of IRS-1 and JAK-2 in the rat pre-optic hypothalamic area in vivo
- Pontos de contato entre as via de transdução evocadas pela noradrenalina e pela angiotensina-II na glândula pineal de ratos
- Insulin temporal sensitivity and its sugnaling pathway on rat pineal gland
- Melatonina induz fosforilação do irs-1 na região pré-optica hipotalâmica anterior de ratas pré-puberes
- Melatonina induz fosforilação de tirosinas do IRS-1 e da JAK-2 na área pré-ópcica hipotalâmica anterior de ratos in vivo
- In vivo activation of insulin receptor tyrosine by melatonin in the rat hypothalamus
- Seasonal pattern of plasma concentrations of melatonin in ewe lambs from santa ines breed, under natural photoperiod in southeast of Brazil
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
