Mycoplasma arthritidis superantigen (MAM)-induced macrophage nitric oxide release is MHC class II restricted, interferongamma (IFNg) dependent and toll-like receptor 4 (TLR4) independent (2003)
- Authors:
- USP affiliated authors: NEGRO, SONIA JANCAR - ICB ; LIMA, FABIO BESSA - ICB ; TIMENETSKY, JORGE - ICB
- Unidade: ICB
- Subjects: IMUNOLOGIA; MICROBIOLOGIA; FISIOLOGIA
- Language: Português
- Abstract: Aims: MAM makes a bridge between lymphocytes T receptor and I-Ealpha MHC class II molecules. Here we studied if MAM-induced nitric oxide (NO) production is H-2 restricted, dependent on the lymphocyte, IFNg or TLR4 signaling. Methods: Resident peritoneal cells (4x105 cells/100 mL) from C3H/HePas (H-2k), C3H/HeJ (H-2k), BALB/c (H-2d), C57BL/6 (H-2b) mice and J774A.1 cells (H-2d - 1x105 cells/100 mL) were co-cultured in the presence or absence of spleen lymphocytes (1:2 ratio) and stimulated with MAM or LPS (1 microg/mL). Nitrite concentration was measured in culture supernatants by Griess reaction. Results: In peritoneal cells from C3H/HePas, C3H/HeJ and BALB/c, maximal NO release was obtained with 1/100 dilution of MAM and 48 h incubation being around 75 microM. Significantly lower levels were found in C57Bl/6 peritoneal cells (18.18 microM). LPS potentiated MAM-induced NO production in C3H/HePas, but not in C3H/HeJ or C57Bl/6 mice (strain non-responsive to LPS or MAM, respectively).Treatment of peritoneal cell with antibody to IFNg inhibited dose-dependently NO production. Depletion of non-adherent cells significantly decreased NO production (60 microM vs 1.04 microM) and addition of IFNgamma potentiated MAM induced NO production. In J774A.1 macrophages MAM induced NO release but only in the presence of viable BALB/c spleen lymphocytes were added (4.24 vs 28.85 microM). Conclusion: NO production induced by MAM is a) restricted toH-2K and H-2d mice strains; b) independent of TLR4 signaling; c) potentiated by LPS; d) dependent on the presence of non-adherent peritoneal cells or viable spleen lymphocytes; e) regulated by IFNg
- Imprenta:
- Publisher: Comissão de Pesquisa do ICB/USP
- Publisher place: São Paulo
- Date published: 2003
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
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ABNT
SHIO, Marina Tiemi et al. Mycoplasma arthritidis superantigen (MAM)-induced macrophage nitric oxide release is MHC class II restricted, interferongamma (IFNg) dependent and toll-like receptor 4 (TLR4) independent. 2003, Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. . Acesso em: 24 abr. 2024. -
APA
Shio, M. T., Negro, S. J., Timenetsky, J., Lima, F. B., Ribeiro-Dias, F., Oliane, A. P. C., & Metran, C. C. (2003). Mycoplasma arthritidis superantigen (MAM)-induced macrophage nitric oxide release is MHC class II restricted, interferongamma (IFNg) dependent and toll-like receptor 4 (TLR4) independent. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP. -
NLM
Shio MT, Negro SJ, Timenetsky J, Lima FB, Ribeiro-Dias F, Oliane APC, Metran CC. Mycoplasma arthritidis superantigen (MAM)-induced macrophage nitric oxide release is MHC class II restricted, interferongamma (IFNg) dependent and toll-like receptor 4 (TLR4) independent. Resumos. 2003 ;[citado 2024 abr. 24 ] -
Vancouver
Shio MT, Negro SJ, Timenetsky J, Lima FB, Ribeiro-Dias F, Oliane APC, Metran CC. Mycoplasma arthritidis superantigen (MAM)-induced macrophage nitric oxide release is MHC class II restricted, interferongamma (IFNg) dependent and toll-like receptor 4 (TLR4) independent. Resumos. 2003 ;[citado 2024 abr. 24 ] - Mycoplasma arthritidis superantigen (MAM) induces iNOS and COX-2 in murine peritoneal cells: modulation by PAF
- Mycoplasma arthrittidis superantigen (MAM) induces nitric oxide (NO) production in murine macrophages by lymphocyte dependent mechanism
- Superantígeno de Mycoplasma arthritidis (MAM) induz a liberação de óxido nítrico (NO) e prostaglandina E2 (PGE2): modulada pelo fator atividor de plaquetas (PAF)
- Mycoplasma arthritidis superantigen (MAM) induces iNOS and COX-2 in murine peritoneal cells: modulation by PAF
- Mycoplasma arthritidis superantigen (MAM)-induced macrophage nitric oxide release is MHC class II restricted, interferon'gama' (IFN'gama') dependent and toll-like receptor 4 (TLR4) independent
- Mycoplasma arthritidis mitogen (MAM) induces release of Nitric Oxide(NO) by mechanisms involving platelet activating factor (PAF) and prostaglandin E2 (PGE2)
- O superantígeno de Mycoplasma arthritidis (MAM) induz a produção de óxido nítrico (NO) em macrófagos dependentes da ativação de linfócitos
- Interferon gama é necessário para a indução de óxido nítrico (NO) pelo superantígeno de mycoplasma arthritidis (MAM) em células peritoniais murinas
- Mycoplasma arthritidis superantigen (MAM)-induced macrophage nitric oxide release is MHC class II restricted, interferon (gama) dependent, and toll-like receptor 4 independent
- PAF is involved in the Mycoplasma arthirtidis superatingen-triggering pathway for iNOS an COX-2 expression in murine peritoneal cells
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