Recognition and repair of cyclobutane pyrimidine dimers by a photolyase-EGFP fusion protein in normal and DNA repair deficient human fibroblasts (2003)
- Authors:
- Autor USP: MENCK, CARLOS FREDERICO MARTINS - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Português
- Abstract: Objectives: CPDs are the main lesion generated in the DNA after UV-C irradiation. In order to investigate the access to CPD by DNA repair proteins and its role to apoptosis in primary human cells, a recombinant adenovirus vector containing a CPD-photolyase fusion protein was generated (Adphr-EGFP). Methods and Results: The vector Adphr-EGFP was employed to study the specific contribution of CPDs lesions in UV-induced apoptosis in primary fibroblasts. Cells infected with the vector present the expression of the gene after 48 hours, and when UV irradiated and maintained in light conditions, the apoptotic cell death was decreased, a phenotypic response that the fusion protein is being functional in CPD elimination. The formation of "foci" of the photolyase fusion protein after local UV was observed 2 minutes after the irradiation, co-localizing with the CPD lesion, the XPC protein and the subunit of the TFIIH factor, p62.In the cells submitted to PRL conditions immediately after UV, a redistribution of the phr-EGFP protein was observed as the appearance of "foci" diffuse, at the same time that the signal of the CPD lesion is decreased. When after UV irradiation the cells are maintained during 1 hour in dark conditions before exposition to light, a different response of the photolyase enzyme in the nucleus is observed in a XPC mutated cell line, as if the phr-EGFP is no more able to perform photorepair. Conclusions: Our data demonstrate invivo the access to CPDs lesions by CPD-photolyase, co-localizing with NER enzymes, with a redistribution of the CPD-photolyase after photorepair of CPDs in vivo. The results in XPC deficient cells indicate a possible competition to CPD access in this cell line
- Imprenta:
- Publisher: Comissão de Pesquisa do ICB/USP
- Publisher place: São Paulo
- Date published: 2003
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
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ABNT
CHIGANÇAS, Vanessa e MENCK, Carlos Frederico Martins e SARASIN, A. Recognition and repair of cyclobutane pyrimidine dimers by a photolyase-EGFP fusion protein in normal and DNA repair deficient human fibroblasts. 2003, Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. . Acesso em: 19 abr. 2024. -
APA
Chiganças, V., Menck, C. F. M., & Sarasin, A. (2003). Recognition and repair of cyclobutane pyrimidine dimers by a photolyase-EGFP fusion protein in normal and DNA repair deficient human fibroblasts. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP. -
NLM
Chiganças V, Menck CFM, Sarasin A. Recognition and repair of cyclobutane pyrimidine dimers by a photolyase-EGFP fusion protein in normal and DNA repair deficient human fibroblasts. Resumos. 2003 ;[citado 2024 abr. 19 ] -
Vancouver
Chiganças V, Menck CFM, Sarasin A. Recognition and repair of cyclobutane pyrimidine dimers by a photolyase-EGFP fusion protein in normal and DNA repair deficient human fibroblasts. Resumos. 2003 ;[citado 2024 abr. 19 ] - Restoring DNA repair capacity of cells from three distinct diseases by XPD gene-recombinant adenovirus
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