Passos iniciais para o estudo da expressão e localização subcelular da protease FtsH-p1 em Arabidopsis thaliana (2004)
- Authors:
- Autor USP: SILVA FILHO, MARCIO DE CASTRO - ESALQ
- Unidade: ESALQ
- Subjects: PROTEÍNAS DE PLANTAS; MELHORAMENTO GENÉTICO VEGETAL
- Language: Português
- Abstract: Despite the central role of FtsH proteases in cellular processes, the expression of these proteins are poorly understood. In this work we propose to study the expression and subcellular localization of FtsH-p1 protease from Arabidopsis thaliana. Material e/ou métodos: Using the genome sequence of the Arabidopsis thaliana available at NCBI, we amplified by PCR the promoter region of the FtsH-p1 as well as a 1365 pb region in located within a previous orf. These sequences were cloned in the vector pCAMBIA-1301. To determine the subcellular localization of the FtsH-p1, a transient expression system in onion cells was carried out. Then, the 5 region encoding the putative targeting sequences followed by two transmembrane segments were fused in frame to the Green Fluorescent Protein (GFP) gene in the pCAMBIA 1302 vector, under the control of the 35S promoter. After bombardment, the subcellular location of the GFP was visualized on a fluorescent microscope. Resultados: The sequences related to promoter region of the FtsH-p1 gene was successfully amplified by PCR and cloned into the pCAMBIA-1301 vector in order to drive the expression of the gus reporter gene in transgenic Arabidopsis plants. GFP fused to the N-terminal region of sugarcane FtsHs-p1 was specifically targeted to the plastids. Conclusões: The study of the protein expression is an important method in the agreement and characterization of genes with potential of use in genetic engineering
- Imprenta:
- Source:
- Título do periódico: Agropecuária; resumos
- Conference titles: Simpósio Internacional de Iniciação Cientifica da Universidade de São Paulo
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ABNT
FIORI, C. S. e MARBACH, P. A. S. e SILVA FILHO, Márcio de Castro. Passos iniciais para o estudo da expressão e localização subcelular da protease FtsH-p1 em Arabidopsis thaliana. 2004, Anais.. São Paulo: USP, 2004. . Acesso em: 19 abr. 2024. -
APA
Fiori, C. S., Marbach, P. A. S., & Silva Filho, M. de C. (2004). Passos iniciais para o estudo da expressão e localização subcelular da protease FtsH-p1 em Arabidopsis thaliana. In Agropecuária; resumos. São Paulo: USP. -
NLM
Fiori CS, Marbach PAS, Silva Filho M de C. Passos iniciais para o estudo da expressão e localização subcelular da protease FtsH-p1 em Arabidopsis thaliana. Agropecuária; resumos. 2004 ;[citado 2024 abr. 19 ] -
Vancouver
Fiori CS, Marbach PAS, Silva Filho M de C. Passos iniciais para o estudo da expressão e localização subcelular da protease FtsH-p1 em Arabidopsis thaliana. Agropecuária; resumos. 2004 ;[citado 2024 abr. 19 ] - Evolutionary history of Arabidopsis thaliana aminoacyl-tRNA synthetase dual targeted proteins
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