Detection of early response genes in rat adrenal stimulated by acth and FGF2 using infusion in situ system (2004)
- Authors:
- Autor USP: LOTFI, CLAUDIMARA FERINI PACICCO - ICB
- Unidade: ICB
- Subjects: ANATOMIA; BIOLOGIA CELULAR
- Language: Inglês
- Abstract: In order to obtain further information on the trophic mechanism of action of ACTH (adrenocorticotropin) and FGF2 (fibroblast growth factor) in an architectural-preserved intact gland system, we used an in situ isolated rat adrenal infusion technique. We analyzed early response genes expression, fos, jun and myc families of proto-oncogenes related with G0- G1-S transition of cell cycle to insight the composition of complex AP-1. Sprague-Dawley male rats were anesthetized and the left adrenal gland was isolated with clamps and the abdominal aorta cannulated. The gland was provided with DMEM/heparin (30min) and then treated (2h) with 10- 7M ACTH(A) or/and 20ng/ml FGF2(F) using an infusion pump (200µl/min). The glands were fixed, embedded in paraffin, cut at 6µm and the protein expression was determined by immunohistochemistry. Without stimulation Fra-2 and c-Jun proteins were detected in medulla but not in the adrenal cortex of control rats. Glands treated with ACTH showed Fra-2 and c-Jun expression in ZF and ZR but not in ZG while FGF2 treatments showed c-Jun protein expression also in ZG. A+F combination showed increase in the c-Jun levels in ZR and medulla but down regulation in ZF. In conclusion ACTH and FGF2 are capable of differentially regulating c-Jun protein and this situation could result in change in the ratio of protein forming the AP-1 complex
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
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ABNT
BACCARO, Rozana Beatriz Franco e LOTFI, Claudimara Ferini Pacicco. Detection of early response genes in rat adrenal stimulated by acth and FGF2 using infusion in situ system. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 19 abr. 2024. -
APA
Baccaro, R. B. F., & Lotfi, C. F. P. (2004). Detection of early response genes in rat adrenal stimulated by acth and FGF2 using infusion in situ system. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Baccaro RBF, Lotfi CFP. Detection of early response genes in rat adrenal stimulated by acth and FGF2 using infusion in situ system. Abstracts. 2004 ;[citado 2024 abr. 19 ] -
Vancouver
Baccaro RBF, Lotfi CFP. Detection of early response genes in rat adrenal stimulated by acth and FGF2 using infusion in situ system. Abstracts. 2004 ;[citado 2024 abr. 19 ] - Involvement of map kinases pathway mediated by acth and FGF2 in rat adrenal primary culture cells
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