Jararhagin stimulates epithelial cell migration, promotes actin polymerization, fak phosphorylation and the recruitment of specific integrins to focal contacts (2004)
- Authors:
- Autor USP: SANTOS, MARINILCE FAGUNDES DOS - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Inglês
- Abstract: Objectives: Jararhagin (JG) is a toxin from the venom of Bothrops jararaca that has different domains such as the metalloprotease and disintegrin. In this study we investigated the effects of JG on epithelial cell adhesion and migration in vitro, using an epithelial restitution experimental model. Methods and results: F-actin arrangement and the distribution of laminin, fibronectin, several integrins and phosphorylated FAK were studied using rodhamine-plalloidin and immunofluorescence. Maximum stimulation of migration (about 100%) was obtained with 5 µg/ml JG, with about 38% inhibition of cellular adhesion. In migratory cells the toxin stimulated the formation of filopodia, lamellipodia and stress fibers. The pericellular fibronectin matrix was lost in migrating cells, while laminin was less affected. The toxin stimulated FAK phosphorylation and the recruitment of ?v-containing integrins to focal contacts, whereas integrins containing the ?2 subunit were reduced in these junctions. Inactivation of the toxin with 1,10 phenantroline showed that the catalytic activity is important for the effect of JG on cell migration, FAK phosphorylation and for the recruitment of ?v, but not as much for the anti-adhesive effect. Conclusion: Jararhagin stimulates the migration of epithelial cells in vitro through a mechanism that involves its proteolytic activity, qualitative changes in cellular adhesion and the formation of actin-rich cellular processes
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
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ABNT
COSTA, Erica Pereira e SANTOS, Marinilce Fagundes dos. Jararhagin stimulates epithelial cell migration, promotes actin polymerization, fak phosphorylation and the recruitment of specific integrins to focal contacts. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 23 abr. 2024. -
APA
Costa, E. P., & Santos, M. F. dos. (2004). Jararhagin stimulates epithelial cell migration, promotes actin polymerization, fak phosphorylation and the recruitment of specific integrins to focal contacts. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Costa EP, Santos MF dos. Jararhagin stimulates epithelial cell migration, promotes actin polymerization, fak phosphorylation and the recruitment of specific integrins to focal contacts. Abstracts. 2004 ;[citado 2024 abr. 23 ] -
Vancouver
Costa EP, Santos MF dos. Jararhagin stimulates epithelial cell migration, promotes actin polymerization, fak phosphorylation and the recruitment of specific integrins to focal contacts. Abstracts. 2004 ;[citado 2024 abr. 23 ] - Hemocitopoese
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