Jararhagin promotes actin polymerization and increases leukocyte adhesion to extracellular matrix and endothelial cells (2004)
- Authors:
- Autor USP: SANTOS, MARINILCE FAGUNDES DOS - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Inglês
- Abstract: Objectives: The snake venom metalloproteinase-disintegrin Jararhagin (JG) has no chemotactic activity but stimulates the migration of epithelial cells in vitro and neutrophils in vivo, through a mechanism still unclear. In this study we analyzed the effects of JG on leukocyte adhesion to several substrata, as well as their filamentous actin (F-actin) organization. Methods and results: Blood samples were collected from Wistar rats and the leukocytes were isolated using a discontinuous gradient of Percoll®. Neutrophils-enriched fractions (98%) were treated with different concentrations of JG (0,31 to 10µg/ml) and submitted to adhesion assays using treated dishes, basement membrane extract (Matrigel®) and non-stimulated murine endothelial cells (tEND) as substrata. Controls consisted of unstimulated cells and cells treated with 1 µM n-formylmethonyl- leucyl-phenylalanine (fMLP). The number of adherent cells was evaluated through myeloperoxidase activity, whereas rodhamine-phalloidin was used for F-actin staining. The toxin increased cellular adhesion to treated dishes and tEND in concentrations above 0,31µg/ml after 30 and 90 minutes, respectively, but not in a concentration-related manner. Adhesion to Matrigel was increased after treatment with 2,5µg/ml JG during 30 minutes. Under the same conditions, JG clearly stimulated cortical actin polymerization in adherent neutrophils. Conclusions: JG promotes actin polymerization and stimulates theadhesion of neutrophils to different substrata
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
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ABNT
COSTA, Erica Pereira e SANTOS, Marinilce Fagundes dos. Jararhagin promotes actin polymerization and increases leukocyte adhesion to extracellular matrix and endothelial cells. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 26 abr. 2024. -
APA
Costa, E. P., & Santos, M. F. dos. (2004). Jararhagin promotes actin polymerization and increases leukocyte adhesion to extracellular matrix and endothelial cells. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Costa EP, Santos MF dos. Jararhagin promotes actin polymerization and increases leukocyte adhesion to extracellular matrix and endothelial cells. Abstracts. 2004 ;[citado 2024 abr. 26 ] -
Vancouver
Costa EP, Santos MF dos. Jararhagin promotes actin polymerization and increases leukocyte adhesion to extracellular matrix and endothelial cells. Abstracts. 2004 ;[citado 2024 abr. 26 ] - Hemocitopoese
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