Effects of hypophysectomy and acth administration on trophic response of rat adrenal gland (2004)
- Authors:
- Autor USP: LOTFI, CLAUDIMARA FERINI PACICCO - ICB
- Unidade: ICB
- Assunto: ANATOMIA
- Language: Inglês
- Abstract: The purpose of this study was to evaluate the effects of hypophysectomy on morphological changes of rat adrenal cortex gland and analyzed the fos, jun and myc protooncogenes families expression in hypophysectomized rats (HP) treated with ACTH (adrenocorticotropin) and/or FGF2 (fibroblast growth factor). A number of studies have demonstrated induction of Fos and Jun protein expression following ACTH and FGF2 treatment of adrenal cells in culture. However, the effect of ACTH and FGF2 on regulation of these protein families in the zones of adrenal cortex (glomerulosa-zG, fasciculata-zF and reticularis-zR) has not been demonstrated in vivo. Furthermore, regulation of these proteins has not been analyzed following alterations in the adrenal tropic state of an animal. Male Sprague Dawley rats were HP according to standard procedures and kept under normal diet. The operation was confirmed by physical inspection of sella turcica. HP received an i.p. injection of either saline, 10-7MACTH or/and 20ng/mlFGF2, 2h prior to harvesting the glands. Animals were killed and the gland harvested, fixed, embedded in paraffin, cut at 5µm. Morphological alterations was measured using HE stain and the protein expression was determined by immunohistochemistry. The atrophic effect of HP was in the inner adrenal cortical zones (zF and zR) and had less effect in zG and was seen within 3 days following HP. HP rats treated with ACTH showed cFos protein expression in zF
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
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ABNT
TORRES, T. E. P. e LOTFI, Claudimara Ferini Pacicco. Effects of hypophysectomy and acth administration on trophic response of rat adrenal gland. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 19 abr. 2024. -
APA
Torres, T. E. P., & Lotfi, C. F. P. (2004). Effects of hypophysectomy and acth administration on trophic response of rat adrenal gland. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Torres TEP, Lotfi CFP. Effects of hypophysectomy and acth administration on trophic response of rat adrenal gland. Abstracts. 2004 ;[citado 2024 abr. 19 ] -
Vancouver
Torres TEP, Lotfi CFP. Effects of hypophysectomy and acth administration on trophic response of rat adrenal gland. Abstracts. 2004 ;[citado 2024 abr. 19 ] - Involvement of map kinases pathway mediated by acth and FGF2 in rat adrenal primary culture cells
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