CuZn-SOD expression and microglia activation in the hypothalamus of Walker-256 tumos-bearing rats (2004)
- Authors:
- USP affiliated authors: BRITTO, LUIZ ROBERTO GIORGETTI DE - ICB ; CURI, RUI - ICB
- Unidade: ICB
- Assunto: FISIOLOGIA
- Language: Inglês
- Abstract: There are large evidences that the Walker-256 tumor (WT-256) induces reactive oxygen species (ROS) production. The neural tissue has intense metabolism and frequently produces ROS, which may be counterbalanced by antioxidant action. The CuZn-SOD removes the superoxide anion (O2o) from the organism. Microglial cells are resident macrophages of the central nervous system (SNC) that when activated can produce ROS. In this study we evaluate the CuZn-SOD expression and the microglia activation by immunohistochemistry (IH) and Western-blotting (WB) in the SNC of WT-256 rats. For this 2x106 cells was subcutaneously injected on twenty rats. The IH was performed after 2 weeks when the brains were removed. Serial coronal brain sections were incubated with CuZn-SOD antibody and the OX-42 antibody that detect the microglial cells. The detection was performed with avidin-biotin peroxidase system (ABC). To WB analysis hypothalamic samples were applied on 10% SDS-PAGE and then nitrocellulose membrane was incubated with the CuZn-SOD antibody and developed. Immunoreactive CuZn-SOD protein expression increased in the hypothalamus of TW rats when evaluated by WB and IH. The IH also demonstrated activated microglial cells in the brain of TW, which can produce ROS and induce the increased expression of CuZn-SOD. These alterations may be related with the central alterations observed in cachetic rats
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
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ABNT
FREITAS, J. J. S. et al. CuZn-SOD expression and microglia activation in the hypothalamus of Walker-256 tumos-bearing rats. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 23 abr. 2024. -
APA
Freitas, J. J. S., Kietzer, K., Britto, L. R. G. de, Anhê, G. F., & Curi, R. (2004). CuZn-SOD expression and microglia activation in the hypothalamus of Walker-256 tumos-bearing rats. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Freitas JJS, Kietzer K, Britto LRG de, Anhê GF, Curi R. CuZn-SOD expression and microglia activation in the hypothalamus of Walker-256 tumos-bearing rats. Abstracts. 2004 ;[citado 2024 abr. 23 ] -
Vancouver
Freitas JJS, Kietzer K, Britto LRG de, Anhê GF, Curi R. CuZn-SOD expression and microglia activation in the hypothalamus of Walker-256 tumos-bearing rats. Abstracts. 2004 ;[citado 2024 abr. 23 ] - Efeitos da aplicação de cloreto de potássio sobre a expressão dos subtipos de receptores muscarínicos da acetilcolina no córtex de ratos
- Atividade da NADPH-d no hipotálamo de ratos portadores de tumor de Walker-256
- Efeitos da aplicação de cloreto de potássio sobre a expressão dos subtipos de receptores muscarínicos da acetilcolina no córtex de ratos
- Ativação de C-FOS no hipotálamo de ratos portadores de tumor de walker-256 (TW)
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