Inoculation of schwann cells pre treated or not with inflammatory cytokines in the contuse lesioned rat spinal cord promoted by the nyu impactor alters the open field motor score (BBB) (2005)
- Authors:
- Autor USP: CHADI, GERSON - ICB
- Unidade: ICB
- Assunto: ANATOMIA
- Language: Português
- Abstract: Objetivo: Schwann cells (SC) plays an important role in neuronal regeneration due the production of neurotrophic molecules, that influence the fibers growth after injury. Inflammatory cytokines induce the mitogenic activity in SC and participate in various nervous system events, contributting for neuronal survival and growth. The aim of the present study was to investigate whether the transforming growth factor beta 1 (TGF-'beta'1) or tumor necrosis factor alpha (TNF-'alfa') can modify the open field motor score (BBB) spinal cord lesioned of rats by the NYU Impactor. Métodos e Resultados: Primary cultured SC were pre-treated with TGF-'beta'1 (5ng/ml) or TNF-'alfa' (30ng/ml) for 30 min or not. Young rats were submitted to a laminectomy (T10-11) and spinal cord received a moderate contuse lesion by means of a 25 cm drop of a 10 g rod promoted by the Impactor. Sham animals received laminectomy without cord lesion. Lesioned rats received injections of SC, with or without pre-treatment with TGF-'beta'1 or TNF-'alfa'. A volume of 10 µl SC (100,000 to 150,000/µl of cultured medium) was injected stereotaxically in the epicenter of the cord lesion immediately after injury. Sham rats received local injection of culture medium alone.The functional recovery of the hindlimbs of the rats was accessed througha open field motor score (BBB) during 8 weeks. Impactor parameters demonstrated the moderate contuse lesion applied in the spinal cord. The BBB showed higher scores in the ratstreated with SC alone 1 week after surgery as well at the 4th, 5th, 6th, 7th and 8th weeks post injury compared with the lesioned rats that were not treated with SC. In the lesioned rats that have received SC pre-treated with TGF-'beta'1 or TNF-'alfa' the BBB presented higher scores only at 2 weeks time-interval. Conclusões: It is concluded that SC inoculation in contuse lesioned spinal cord improves hindlimb motor function. The manipulation of the SC in order to increase the ability of the cells to promote functional recoveryin spinal cord lesioned animal needs to be further investigated.
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
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ABNT
GUZEN, F. P. et al. Inoculation of schwann cells pre treated or not with inflammatory cytokines in the contuse lesioned rat spinal cord promoted by the nyu impactor alters the open field motor score (BBB). 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 19 abr. 2024. -
APA
Guzen, F. P., Taneda, M., De Luca, B. A., Cunha, J. C., Andrade, M. S. R., Gomide, V. C., & Chadi, G. (2005). Inoculation of schwann cells pre treated or not with inflammatory cytokines in the contuse lesioned rat spinal cord promoted by the nyu impactor alters the open field motor score (BBB). In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Guzen FP, Taneda M, De Luca BA, Cunha JC, Andrade MSR, Gomide VC, Chadi G. Inoculation of schwann cells pre treated or not with inflammatory cytokines in the contuse lesioned rat spinal cord promoted by the nyu impactor alters the open field motor score (BBB). Resumos. 2005 ;[citado 2024 abr. 19 ] -
Vancouver
Guzen FP, Taneda M, De Luca BA, Cunha JC, Andrade MSR, Gomide VC, Chadi G. Inoculation of schwann cells pre treated or not with inflammatory cytokines in the contuse lesioned rat spinal cord promoted by the nyu impactor alters the open field motor score (BBB). Resumos. 2005 ;[citado 2024 abr. 19 ] - On the presence of the bfgf immunoreactivity in the spinal cord and dorsal root ganglia of the adult rat
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