Role of reactive oxygen species, nitric oxide synthase enzyme and angiotensin AT1 receptor in male and female spontaneously hypertensive rats treated with losartan (2005)
- Authors:
- USP affiliated authors: PASSAGLIA, RITA DE CASSIA ALEIXO TOSTES - ICB ; SCAVONE, CRISTOFORO - ICB ; CARVALHO, MARIA HELENA CATELLI DE - ICB ; NIGRO, DOROTHY - ICB ; FORTES, ZULEICA BRUNO - ICB
- Unidade: ICB
- Assunto: FARMACOLOGIA
- Language: Português
- Abstract: Objetivo: We demonstrated that losartan corrects the impaired endothelium-dependent vasodilation in mesenteric arterioles more easily in female than in male spontaneously hypertensive rats (SHR), (J Hypertension, 22:S176, 2004). Our objective was to investigate the role of oxygen reactive species (ROS), nitric oxide synthase (NOS) activity and angiotensin AT1 receptor expression on this difference. Métodos e Resultados: Male and female SHR (14-16 weeks) were divided into 03 groups (3-7 animals each) treated or not with losartan for 24 hours or 15 days (15mg/kg/day). After anesthesia (chloral hydrate, 500mg/kg, s.c.), the mesentery was exposed for microscopic observation and ROS generation was studied using the hydroethidine method. NOS activity in the mesentery vascular bed was evaluated using radiolabelled L-[3H]arginine conversion to L-[3H]citrulline (pmol/mg.min). mRNA and protein expressions for AT1-receptor in the mesentery bed were evaluated by RT-PCR and Westernblotting method, respectively. Losartan treatment for 24 h did not diminish ROS (in % ethidium bromide positive nuclei) in male (19±1.5% untreated vs 17.8±1.9% 24h) or female (11.4±1.5% untreated vs 14.9±2.0% 24h) SHR. Chronic or acute losartan treatment did not alter NOS activity in male (5.6±0.8 untreated vs 7.0±0.3 24h vs 3.9±0.4 15d) or female (5.9±0.8 untreated vs 6.2±0.3 24h vs 4.3±0.8 15d) SHR. Gene or protein AT1-receptor expressions were not modified by losartan treatment either in male orfemale SHR. Conclusões: Thus, differences in ROS generation, NOS activity or angiotensin AT1 receptor expression could not account for the differences in the responses of male and female SHR to losartan treatment.
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
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ABNT
RODRIGUES, Stephen Fernandes de Paula et al. Role of reactive oxygen species, nitric oxide synthase enzyme and angiotensin AT1 receptor in male and female spontaneously hypertensive rats treated with losartan. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 20 abr. 2024. -
APA
Rodrigues, S. F. de P., Eichler, R. A. S., Urakawa, T. A. E., Silva-Antonialli, M. M., Scavone, C., Carvalho, M. H. C. de, et al. (2005). Role of reactive oxygen species, nitric oxide synthase enzyme and angiotensin AT1 receptor in male and female spontaneously hypertensive rats treated with losartan. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Rodrigues SF de P, Eichler RAS, Urakawa TAE, Silva-Antonialli MM, Scavone C, Carvalho MHC de, Nigro D, Passaglia R de CAT, Fortes ZB. Role of reactive oxygen species, nitric oxide synthase enzyme and angiotensin AT1 receptor in male and female spontaneously hypertensive rats treated with losartan. Resumos. 2005 ;[citado 2024 abr. 20 ] -
Vancouver
Rodrigues SF de P, Eichler RAS, Urakawa TAE, Silva-Antonialli MM, Scavone C, Carvalho MHC de, Nigro D, Passaglia R de CAT, Fortes ZB. Role of reactive oxygen species, nitric oxide synthase enzyme and angiotensin AT1 receptor in male and female spontaneously hypertensive rats treated with losartan. Resumos. 2005 ;[citado 2024 abr. 20 ] - In vivo evidence of oxidative stress in intrauterine programming of hypertensive in wistar rats
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