Initial peptide content characterization in mammalian tissues using LC-ESI-MS/MS (2005)
- Authors:
- Autor USP: FERRO, EMER SUAVINHO - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Inglês
- Abstract: Objectives Proteasomal proteolyses produces large quantities of intracellular peptides from natural misfolded or aged proteins. Some evidences suggest the involvement of such intracellular peptides as modulators of protein interactions. Therefore, we developed a new methodology that uses inactive oligopeptidases to identify specific peptides in complex biological samples. The goal of the present work was to use LC-ESI-MS/MS to identify and sequence these complex peptide mixtures obtained from filtrates of rodentia tissues.Methods and Results Briefly, tissues were homogenized in 0,05 M HCl and centrifuged at 100.000 x g for 60 min, at 4 C. The supernatants were subjected to size exclusion using a Millipore filter device (Millipore, USA). The resulting material containing peptides smallest than 5 kDa were concentrated using a 1 cc OASIS HLB (Waters, UK) and filtrated using the inactive oligopeptidases. LC-ESI-MS/MS analysis were performed at the Mass Spectrometry Laboratory, National Laboratory of Light Sincrotron (LNLS), Campinas, SP, in a Q-Tof (Waters, UK) coupled to a capillary liquid chromatography system (CapLC Waters). Thirty nine peptides were sequenced in samples from adipose (rat and mouse), brain and spleen (rat) tissues, and match internal regions of known and unknown putative intracellular proteins. Organizing these peptides based on their putative motifs we found that 33% contain protein kinase (PK) C; 23% PKA; 10% C kinase 2 (CK2); 3% C kinase 1 (CK1);05% P38 mitogenactivating PK (MAPK) phosphorylation sites, and 3% contained a glycosilation site. The remaining 9 peptides contained no putative motifs. Conclusion These initial results suggest that most of peptides identified herein contain phosphorylation sites and could be modulating intracellular signaling
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
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ABNT
BERTI, Denise Aparecida e KLITZKE, C. F. e FERRO, Emer Suavinho. Initial peptide content characterization in mammalian tissues using LC-ESI-MS/MS. 2005, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005. . Acesso em: 19 abr. 2024. -
APA
Berti, D. A., Klitzke, C. F., & Ferro, E. S. (2005). Initial peptide content characterization in mammalian tissues using LC-ESI-MS/MS. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Berti DA, Klitzke CF, Ferro ES. Initial peptide content characterization in mammalian tissues using LC-ESI-MS/MS. Resumos. 2005 ;[citado 2024 abr. 19 ] -
Vancouver
Berti DA, Klitzke CF, Ferro ES. Initial peptide content characterization in mammalian tissues using LC-ESI-MS/MS. Resumos. 2005 ;[citado 2024 abr. 19 ] - Secretion of metalloendopeptidase 24.15 (EC 3.4.24.15)
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