Regulation of TH1/2 differentiation is controlled by p38 map kinase and MSK1/2 circuit (2006)
- USP affiliated authors: CAMARGO, MARISTELA MARTINS DE - ICB
- USP Schools: ICB
- Subjects: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: Differentiation of CD4+ T lymphocytes into functional Th1 and Th2 is characterized by production of hallmark cytokines, IFN-g and IL-4, respectively. While transcription factors T-bet and GATA-3 and chromatin remodelling are required for the acquision/maintenance of the ability to transcribe those cytokines, little is known about the signaling pathways regulating these events. Here in we aimed at uncovering the signaling cascades involved in the early phase of Th1/2 differentiation. Methods and Results: Human CD4+ lymphocytes were differentiated in vitro into functional Th1/2 for 6 days. Production of IFN-g and IL-4 was assayed by intracellular cytokine staining and flow cytometric analysis. The presence of 10mM SB203580, a specific inhibitor of p38 MAP kinase, resulted in skewed differentiation favoring Th2, regardless of whether the differentiation condition favored Th1 or Th2.To assess the participation of MSK, we performed RNAi silencing of MSK1 in CD4+ cells prior to differentiation. The number of cells committed to IFN-g production decreased (30 to 10%) and those committed to IL-4 production increased (6 to 33%) even under Th1-inducing condition, mirroring the results with SB203580. Furthermore, differentiation of CD4+ cells from mice deficient of MSK1/2 was comparable to RNAi silencing. Only 3% of MSK1/2-/- cells committed to IFN-g production compared to wild-type controls that presented8.3% of cells committed to Th1. Similarly to results with human cells, an increase in the number of Th2 cells was observed under Th1-inducing conditions. Conclusion: Our data suggest that p38 MAP kinase and its downstream targets, MSK1/2 are involved in the Th1/2 differentiation process, playing opposite roles in the regulation of IFN-g and IL-4 production.
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
ABNTBOMBARDIERI, C R; KURIBAYASHI, J S; COHEN, P; ARTHUR, S; CAMARGO, Maristela Martins de. Regulation of TH1/2 differentiation is controlled by p38 map kinase and MSK1/2 circuit. Anais.. São Paulo: [s.n.], 2006.
APABombardieri, C. R., Kuribayashi, J. S., Cohen, P., Arthur, S., & Camargo, M. M. de. (2006). Regulation of TH1/2 differentiation is controlled by p38 map kinase and MSK1/2 circuit. In Abstracts. São Paulo.
NLMBombardieri CR, Kuribayashi JS, Cohen P, Arthur S, Camargo MM de. Regulation of TH1/2 differentiation is controlled by p38 map kinase and MSK1/2 circuit. Abstracts. 2006 ;
VancouverBombardieri CR, Kuribayashi JS, Cohen P, Arthur S, Camargo MM de. Regulation of TH1/2 differentiation is controlled by p38 map kinase and MSK1/2 circuit. Abstracts. 2006 ;
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