IL-2 and 'T IND.REG' cells in polyclonal lymphocyte activation (PLA) induced by Plasmodium chabaudi infection (2006)
- Authors:
- USP affiliated authors: ABRAHAMSOHN, ISES DE ALMEIDA - ICB ; MOSIG, JOSE MARIA ALVAREZ - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: IL-2 was described as an important T cell growth factor, which also shows apoptotic activity. Recently, IL-2 has been implicated in CD4+CD25+ (Treg) cell generation and maintenance, being responsible for self-tolerance and autoimmune disease control. PLA and autoantibody production are common features of the m alaria immune response. As PLA includes CD4+ T cells that secrete cytokines and help antibody production, we have analyzed IL-2 secretion and IL-2R expression at the first days of P. chabaudi infection, and also the effects of anti-IL-2 treatment on the different CD4+ T cell subsets. Methods and Results: IL-2 secretion by CD4+ T cells was revealed by the Cytokine Secretion Assay (PharMingen) in C57BL/6 mice infected with 106 P. chabaudi parasitized erythrocytes. It began at day 5 of infection and remained until the 7th day.Among CD4+ T cells, large cells expressing CD25 and CD122 (? and ? chains of IL-2R, respectively) were the major source of IL-2. These cells also expressed very high levels of CTLA-4, mTGF-?, GITR and CD45RB. We also observed a Treg celllike population, i.e., small lymphocytes with high levels of Foxp3, CTLA-4, mTGF-?, GITR and low levels of CD45RB and CD122. Moreover the number of Foxp3+ cells remained unchanged on day 7 of infection (around 9.0%). In order to evaluate the role of IL-2 in the polyclonal CD4+ T cell response, mice were treated with anti-IL-2 mAbs (S4B6) on days0, 2 and 4 after infection. At day 7 of infection, these mice showed significantly higher frequencies of large CD4+ T cells expressing CD44, CD69 and CD122 (a part also expressing CD25), when compared to untreated infected mice. However, anti-IL-2 treatment led to a reduction in the percentage of Foxp3+ cells, from 9.0% to 3.9% in non-infected mice and from 9.4% to 3.9% in 7 daysinfected mice. Conclusions: Our findings suggest that IL-2 secreted by activated CD4+ T cells during acute P. chabaudi infection is not essential for CD4+ T cell proliferation, but it is required for maintaining Treg cell activity.
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
ZAGO, C et al. IL-2 and 'T IND.REG' cells in polyclonal lymphocyte activation (PLA) induced by Plasmodium chabaudi infection. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 12 maio 2024. -
APA
Zago, C., Bastos, K. R. B., Sardinha, L. R., Pretel, F. D., Castillo, S. I., Abrahamsohn, I. de A., et al. (2006). IL-2 and 'T IND.REG' cells in polyclonal lymphocyte activation (PLA) induced by Plasmodium chabaudi infection. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Zago C, Bastos KRB, Sardinha LR, Pretel FD, Castillo SI, Abrahamsohn I de A, Alvarez JM, Lima MRD'I. IL-2 and 'T IND.REG' cells in polyclonal lymphocyte activation (PLA) induced by Plasmodium chabaudi infection. Abstracts. 2006 ;[citado 2024 maio 12 ] -
Vancouver
Zago C, Bastos KRB, Sardinha LR, Pretel FD, Castillo SI, Abrahamsohn I de A, Alvarez JM, Lima MRD'I. IL-2 and 'T IND.REG' cells in polyclonal lymphocyte activation (PLA) induced by Plasmodium chabaudi infection. Abstracts. 2006 ;[citado 2024 maio 12 ] - TCR'a''b'/CD8 down-regulated CD8+ cells in the spleen of Trypanosoma cruzi - Infected chronic mice are responsive to in vitro and in vivo stimulation with the parasite
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