Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain (2013)
- USP affiliated authors: LOPES, MARILENE HOHMUTH - ICB
- USP Schools: ICB
- Subjects: BIOLOGIA CELULAR; BIOLOGIA DO DESENVOLVIMENTO; CELULAS-TRONCO; PLASTICIDADE NEURAL; SISTEMA NERVOSO CENTRAL
- Language: Português
- Abstract: Introdução The ocurrence of neurogenesis in adult brain was disbelieved for several years. Nowadays, it is recognized the existence of neurogenic niches in mammalian adult brain, sites where new neurons are generated throughout life, being the best studied the ventricular-subventricular zone (V-SVZ) and subgranular zone (SGZ). These niches harboring adult multipotent neural stem cells (adNSCs) that give rise to new neurons and glial cells. While science has evolved into acceptance of neurogenesis, important studies have reported the cellular prion protein (PrPC), a membrane glycoprotein highly expressed in the central nervous system (CNS) and widely conserved during evolution, as an important factor in neurogenesis and biology of adNSCs. Steele and colleagues reported the PrPC as important factor in neurogenesis during development and adulthood. Moreover, our group identified as PrPC ligand, the co-chaperone called stress-inducible protein one (STI1), which interaction promotes neuritogenesis, neuroprotection, formation of short-term memory (STM), long-term memory (LTM) consolidation and self-renewal of fetal neural stem cells. Given these findings, it becomes important to know if the PrPC-STI1 complex also acts similarly in adNSCs. This study provide a better understanding of mechanisms that mediate neurogenesis and plasticity in the adult CNS, as well as novel strategies for the treatment of neurological disorders. Objetivos To evaluate the role of the PrPC-STI1 complex in the self-renewal, proliferation and differentiation of adNSCs from V-SVZ murine brain.Métodos Neurosphere primary cultures of adNSCs derived from the V-SVZ from wild-type adult animals ( PrPn +/+) or knockout ( Prpn0/0) for PrPC have been performed in the presence of absence of recombinant STI1 in self-renewal or proliferation assays. This study was approved by Animal Ethic Committe from A.C. Camargo Hospital (protocol 025/08). Resultados Preliminary results indicate that STI1 is able to promote self-renewal and proliferation in murine adNSCs in a PrPC-dependent manner. Conclusão Additional experiments should be performed to confirm the participation of PrPC-STI1 complex upon proliferation and self-renewal of adNSCs.
- Título do periódico: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental (FeSBE)
ABNTDA SILVA, C. M. C.; SANTOS, T. G.; MARTINS, V. R.; LOPES, Marilene Hohmuth. Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain. Anais.. Caxambu: FeSBE, 2013.
APADa Silva, C. M. C., Santos, T. G., Martins, V. R., & Lopes, M. H. (2013). Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain. In Resumos. Caxambu: FeSBE.
NLMDa Silva CMC, Santos TG, Martins VR, Lopes MH. Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain. Resumos. 2013 ;
VancouverDa Silva CMC, Santos TG, Martins VR, Lopes MH. Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain. Resumos. 2013 ;
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