Taxonomic relationships among Brazilian Culex (Diptera: Culicidae) mosquitoes inferred from the second internal transcribed spacer of the ribossomal (2008)
- Authors:
- Autor USP: SALLUM, MARIA ANICE MUREB - FSP
- Unidade: FSP
- Subjects: DIPTERA; CULEX (VARIABILIDADE;MORFOLOGIA); FILOGENIA (ANÁLISE GENÉTICA); DNA; VARIAÇÃO GENÉTICA; BIOLOGIA MOLECULAR
- Language: Inglês
- Abstract: The genus Culex is one of the largest genera of Culicidae and include vectors of several arboviruses and filarial worms. Despite that, the knowledge on its taxonomy and phylogeny is still scarce. The identification of the mosquitoes is generally made on the basis of morphological characters. However, many species of Culex are morphologically similar, making it difficult the correct identification. Currently, molecular techniques have been used in both evolution studies and species distinction, including those that belong to complexes. In this study, Cx. (Cux.) bidens, Cx. (Cux.) chidesteri, Cx. (Cux.) coronator, Cx. (Cux.) dolosus, Cx. (Cux.) mollis, Cx. (Mcx.) imitator, Cx. (Mel.) zeteki and Cx. (Mel.) aliciae, collected in several localities in Brazil, were identified based on morphological characteristics of the immature and adults. Sequences of the ITS2 of the rDNA of these species were generated to verify the level of inter-specific differentiation and their relationship with the morphological characteristics. A preliminary analysis was made on the basis of the size of this spacer after electrophoresis and visualization of the products. No differentiation in the size of the fragments among the species of the subgenera Melanoconion and Microculex was found, showing bands of the shortest length (~350bp). The species of the Culex subgenus showed the most variable and largest sizes (from ~450bp to ~500bp), with the largest ITS2 of Cx. (Cux.) coronator. The direct sequencing of the PCR products generated in most cases ambiguous data, indicating intra-individual variation. In this regards, the next step will be to clone the ITS2 PCR products to verify the degree of intra-genomic variation and the possibility of using the ITS2 sequence data for evolutionary studies on the Neotropical Culex subgenera
- Imprenta:
- Publisher: SEB
- Publisher place: Uberlândia
- Date published: 2008
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Brasileiro de Entomologia
-
ABNT
VESGUEIRO, Fabiana Tavares et al. Taxonomic relationships among Brazilian Culex (Diptera: Culicidae) mosquitoes inferred from the second internal transcribed spacer of the ribossomal. 2008, Anais.. Uberlândia: SEB, 2008. Disponível em: http://www.seb.org.br/eventos/cbe/xxiicbe/resumos/R2455-2.html. Acesso em: 28 mar. 2024. -
APA
Vesgueiro, F. T., Silva, B. D. e, Sallum, M. A. M., & Marrelli, M. T. (2008). Taxonomic relationships among Brazilian Culex (Diptera: Culicidae) mosquitoes inferred from the second internal transcribed spacer of the ribossomal. In Resumos. Uberlândia: SEB. Recuperado de http://www.seb.org.br/eventos/cbe/xxiicbe/resumos/R2455-2.html -
NLM
Vesgueiro FT, Silva BD e, Sallum MAM, Marrelli MT. Taxonomic relationships among Brazilian Culex (Diptera: Culicidae) mosquitoes inferred from the second internal transcribed spacer of the ribossomal [Internet]. Resumos. 2008 ;[citado 2024 mar. 28 ] Available from: http://www.seb.org.br/eventos/cbe/xxiicbe/resumos/R2455-2.html -
Vancouver
Vesgueiro FT, Silva BD e, Sallum MAM, Marrelli MT. Taxonomic relationships among Brazilian Culex (Diptera: Culicidae) mosquitoes inferred from the second internal transcribed spacer of the ribossomal [Internet]. Resumos. 2008 ;[citado 2024 mar. 28 ] Available from: http://www.seb.org.br/eventos/cbe/xxiicbe/resumos/R2455-2.html - Memórias do Instituto Oswaldo Cruz
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