Protease-activated receptor 1 (PAR1) enhances ectopic bone formation in human periodontal ligament stem cell (hPDLSC) sheets in vivo (2021)
- Authors:
- USP affiliated authors: SIPERT, CARLA RENATA - FO ; CALDEIRA, MARINELLA HOLZHAUSEN - FO
- Unidade: FO
- Subjects: CÉLULAS-TRONCO; LIGAMENTO PERIODONTAL
- Language: Português
- Abstract: We evaluated the osteogenic outcomes underlying PAR1 activation and how it affects bone formation in hPDLSC sheets in vivo. Briefly, hPDLSCs were obtained from 3 patients. Groups were: control, osteogenic and osteogenic + PAR1 activation by TFLLR-NH2 for 2, 7 and 14 days. PAR1 osteogenic potential pathways Wnt/β-catenin, TGF-βRI, MEK, p38 MAPK, and FGFR/VEGFR were assessed after blockage. Cell phenotype was determined by flow cytometry and immunofluorescence. Colonyforming-unit and cell proliferation assays were performed. qRT-PCR for Alkaline Phosphatase (ALP), β-galactosidase, Collagen I (COL1), Osteocalcin (OC), Osteoprotegerin (OPG), Osterix, PAR1, Periostin (POSTN), RANKL and RUNX2 was conducted. ELISA was performed to COL1, OC, OPG, PAR1, POSTN and RANKL. Calcium deposition was quantified through ALP and Alizarin Red Staining. Cell sheet microstructure was analyzed through H&E, light and scanning electron microscopy. In vivo, 14-day pre-induced hPDLSC sheets from the 3 groups were transplanted to Balb/c nude mice and evaluated after 60 days. Immunohistochemistry for bone sialoprotein (BSP), integrin β1, COL1 and histological stains (H&E, Van Giesson, Masson's Trichrome and Von Kossa) was carried out. Ectopic bone formation was evaluated through micro-CT. One-way ANOVA followed by Tukey's test (P value of 0.05) was used. PAR1 activation significantly increased pro-osteogenic gene and protein expression, calcium deposition and enhanced ectopic bone formation in vivo
- Imprenta:
- Source:
- Título do periódico: Brazilian Oral Research
- ISSN: 1807-3107
- Volume/Número/Paginação/Ano: v. 35, supl. 3, p. 76, res. HA017, 2021
- Conference titles: Proceedings of the SBPqO Annual Meeting
-
ABNT
ALVES, T et al. Protease-activated receptor 1 (PAR1) enhances ectopic bone formation in human periodontal ligament stem cell (hPDLSC) sheets in vivo. Brazilian Oral Research. São Paulo: SBPqO. . Acesso em: 11 jun. 2024. , 2021 -
APA
Alves, T., Gasparoni, L. M., Balzarini. D,, Albuquerque-Souza, E., Rovai, E. S., Mendoza, A. A. H., et al. (2021). Protease-activated receptor 1 (PAR1) enhances ectopic bone formation in human periodontal ligament stem cell (hPDLSC) sheets in vivo. Brazilian Oral Research. São Paulo: SBPqO. -
NLM
Alves T, Gasparoni LM, Balzarini. D, Albuquerque-Souza E, Rovai ES, Mendoza AAH, Sipert CR, Caldeira MH. Protease-activated receptor 1 (PAR1) enhances ectopic bone formation in human periodontal ligament stem cell (hPDLSC) sheets in vivo. Brazilian Oral Research. 2021 ; 35 76.[citado 2024 jun. 11 ] -
Vancouver
Alves T, Gasparoni LM, Balzarini. D, Albuquerque-Souza E, Rovai ES, Mendoza AAH, Sipert CR, Caldeira MH. Protease-activated receptor 1 (PAR1) enhances ectopic bone formation in human periodontal ligament stem cell (hPDLSC) sheets in vivo. Brazilian Oral Research. 2021 ; 35 76.[citado 2024 jun. 11 ] - Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells
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